Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 14646..15247 Replicon plasmid unnamed3
Accession NZ_CP032362
Organism Lactiplantibacillus plantarum strain ZFM55

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag CFM86_RS16545 Protein ID WP_001748110.1
Coordinates 14903..15247 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag CFM86_RS16540 Protein ID WP_001748109.1
Coordinates 14646..14909 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFM86_RS16505 9866..10558 - 693 WP_057813569.1 Fic family protein -
CFM86_RS16510 10574..10966 - 393 WP_119724065.1 DUF536 domain-containing protein -
CFM86_RS16520 11374..11754 - 381 Protein_11 DUF536 domain-containing protein -
CFM86_RS16525 11747..12682 - 936 WP_057813564.1 replication initiation protein -
CFM86_RS16530 13401..13613 - 213 Protein_13 hypothetical protein -
CFM86_RS16535 13973..14560 - 588 WP_119724060.1 site-specific integrase -
CFM86_RS16540 14646..14909 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CFM86_RS16545 14903..15247 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CFM86_RS16550 15501..15782 - 282 WP_080241820.1 IS3 family transposase -
CFM86_RS16555 16239..16856 + 618 WP_021356089.1 L,D-transpeptidase -
CFM86_RS16560 17437..17925 + 489 WP_119724061.1 hypothetical protein -
CFM86_RS16565 18630..19007 + 378 WP_119724062.1 YxeA family protein -
CFM86_RS16570 19238..19720 + 483 WP_016511793.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..43920 43920


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T111706 WP_001748110.1 NZ_CP032362:14903-15247 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T111706 NZ_CP032362:14903-15247 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATTTAGTCTGTCAGACGAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACGGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT111706 WP_001748109.1 NZ_CP032362:14646-14909 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT111706 NZ_CP032362:14646-14909 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References