Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 158369..159108 Replicon plasmid p15WZ-82_res
Accession NZ_CP032357
Organism Klebsiella variicola strain 15WZ-82

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag D4N21_RS30290 Protein ID WP_013087172.1
Coordinates 158369..158854 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag D4N21_RS30295 Protein ID WP_012540086.1
Coordinates 158842..159108 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N21_RS30240 153856..154107 + 252 WP_128206252.1 DUF2274 domain-containing protein -
D4N21_RS30250 154611..154835 - 225 WP_128206253.1 hypothetical protein -
D4N21_RS30255 154846..155058 - 213 WP_128206254.1 hypothetical protein -
D4N21_RS30260 155119..155448 - 330 WP_164875724.1 hypothetical protein -
D4N21_RS30265 155460..155672 - 213 WP_047722603.1 hypothetical protein -
D4N21_RS30270 156539..156856 - 318 WP_032747192.1 hypothetical protein -
D4N21_RS30275 156871..157221 - 351 WP_042938321.1 hypothetical protein -
D4N21_RS30280 157218..157490 - 273 WP_114268495.1 hypothetical protein -
D4N21_RS30285 157868..158029 - 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
D4N21_RS30290 158369..158854 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
D4N21_RS30295 158842..159108 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
D4N21_RS30300 159408..159731 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
D4N21_RS30305 159733..160446 + 714 WP_012540184.1 arsenical resistance protein ArsH -
D4N21_RS30310 160455..161000 + 546 Protein_177 sigma-70 family RNA polymerase sigma factor -
D4N21_RS30315 161076..161438 + 363 WP_012540155.1 arsenic metallochaperone ArsD family protein -
D4N21_RS30320 161465..163216 + 1752 WP_162866166.1 arsenical pump-driving ATPase -
D4N21_RS30325 163341..163877 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..185478 185478


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T111701 WP_013087172.1 NZ_CP032357:c158854-158369 [Klebsiella variicola]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T111701 NZ_CP032357:c158854-158369 [Klebsiella variicola]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT111701 WP_012540086.1 NZ_CP032357:c159108-158842 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT111701 NZ_CP032357:c159108-158842 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCCAAGGAATCACAGCGGGCGCTCATTGACGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAATGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References