Detailed information of TA system TA111698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 184553..185304
• Replicon: plasmid p15WZ-82_Vir
• Accession: NZ_CP032356
• Organism: Klebsiella variicola strain 15WZ-82

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: D4N21_RS28860
• Protein ID: WP_048333033.1
• Coordinates: 184553..185035 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: D4N21_RS28865
• Protein ID: WP_004902250.1
• Coordinates: 185026..185304 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N21_RS28830	180952..181599	-	648	WP_014386537.1	EcsC family protein	-
D4N21_RS28835	181626..182381	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
D4N21_RS28840	182482..182874	-	393	WP_123812462.1	hypothetical protein	-
D4N21_RS28845	182979..183518	-	540	WP_004902239.1	hypothetical protein	-
D4N21_RS28860	184553..185035	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
D4N21_RS28865	185026..185304	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
D4N21_RS28870	185426..185638	-	213	WP_016946198.1	hypothetical protein	-
D4N21_RS28875	185746..186087	-	342	WP_032437747.1	hypothetical protein	-
D4N21_RS28890	186993..187307	+	315	WP_064190282.1	hypothetical protein	-
D4N21_RS28895	187551..188033	-	483	WP_004210306.1	hypothetical protein	-
D4N21_RS28900	188505..189440	-	936	WP_004026564.1	omptin family outer membrane protease Kop	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..282290	282290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T111698 WP_048333033.1 NZ_CP032356:c185035-184553 [Klebsiella variicola]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T111698 NZ_CP032356:c185035-184553 [Klebsiella variicola]
ATGGGAATGAGAGCGCCTGAATCCCTGATGTCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGAAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT111698 WP_004902250.1 NZ_CP032356:c185304-185026 [Klebsiella variicola]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT111698 NZ_CP032356:c185304-185026 [Klebsiella variicola]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACACCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3