Detailed information of TA system TA111697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 141076..141746
• Replicon: plasmid p15WZ-82_Vir
• Accession: NZ_CP032356
• Organism: Klebsiella variicola strain 15WZ-82

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: D4N21_RS28620
• Protein ID: WP_004213072.1
• Coordinates: 141076..141519 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: D4N21_RS28625
• Protein ID: WP_004213073.1
• Coordinates: 141516..141746 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N21_RS28570	136488..136763	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
D4N21_RS28575	136826..137317	+	492	WP_011251327.1	DM13 domain-containing protein	-
D4N21_RS28580	137366..138286	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
D4N21_RS28590	138377..138783	+	407	Protein_159	GAF domain-containing protein	-
D4N21_RS31245	139298..139932	-	635	Protein_160	response regulator	-
D4N21_RS28610	140349..140642	+	294	Protein_161	transposase	-
D4N21_RS28615	140646..141062	+	417	Protein_162	restriction endonuclease	-
D4N21_RS28620	141076..141519	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
D4N21_RS28625	141516..141746	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D4N21_RS28630	142354..143487	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
D4N21_RS28635	143503..143796	+	294	WP_004213076.1	hypothetical protein	-
D4N21_RS28640	143786..143992	-	207	WP_004213077.1	hypothetical protein	-
D4N21_RS28645	144344..144634	+	291	WP_004213078.1	hypothetical protein	-
D4N21_RS28650	144624..145523	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..282290	282290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T111697 WP_004213072.1 NZ_CP032356:c141519-141076 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T111697 NZ_CP032356:c141519-141076 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT111697 WP_004213073.1 NZ_CP032356:c141746-141516 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT111697 NZ_CP032356:c141746-141516 [Klebsiella variicola]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620