Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 37841..38751 Replicon plasmid p15WZ-82_Vir
Accession NZ_CP032356
Organism Klebsiella variicola strain 15WZ-82

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag D4N21_RS28025 Protein ID WP_004026354.1
Coordinates 38281..38751 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag D4N21_RS28020 Protein ID WP_004026357.1
Coordinates 37841..38284 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N21_RS28000 34042..34449 - 408 WP_101415802.1 IS200/IS605 family transposase -
D4N21_RS28005 34506..35693 + 1188 WP_101415803.1 transposase -
D4N21_RS28010 36047..36469 + 423 WP_004197884.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
D4N21_RS28015 36469..37740 + 1272 WP_128206158.1 translesion error-prone DNA polymerase V subunit UmuC -
D4N21_RS28020 37841..38284 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
D4N21_RS28025 38281..38751 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
D4N21_RS28030 38862..39122 - 261 WP_004026352.1 hypothetical protein -
D4N21_RS28040 39846..40004 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
D4N21_RS28045 40383..40625 - 243 WP_128206159.1 hypothetical protein -
D4N21_RS31235 40701..40865 - 165 WP_011154586.1 hypothetical protein -
D4N21_RS31240 40982..41143 - 162 WP_164875713.1 hypothetical protein -
D4N21_RS31390 41533..42507 + 975 WP_185938743.1 Hok/Gef family protein -
D4N21_RS28060 42574..42909 + 336 WP_128206160.1 hypothetical protein -
D4N21_RS28065 43171..43563 + 393 WP_069127090.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla 1..282290 282290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T111695 WP_004026354.1 NZ_CP032356:38281-38751 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T111695 NZ_CP032356:38281-38751 [Klebsiella variicola]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACTCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT111695 WP_004026357.1 NZ_CP032356:37841-38284 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT111695 NZ_CP032356:37841-38284 [Klebsiella variicola]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References