Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2239821..2240411 Replicon chromosome
Accession NZ_CP032354
Organism Klebsiella variicola strain 15WZ-82

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag D4N21_RS11055 Protein ID WP_008804165.1
Coordinates 2240079..2240411 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag D4N21_RS11050 Protein ID WP_012541132.1
Coordinates 2239821..2240078 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N21_RS11025 2235420..2236005 + 586 Protein_2081 hypothetical protein -
D4N21_RS11030 2236183..2237019 + 837 WP_128205796.1 alpha/beta hydrolase -
D4N21_RS11035 2237225..2238196 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
D4N21_RS11040 2238193..2239293 - 1101 Protein_2084 phosphotransferase -
D4N21_RS11050 2239821..2240078 + 258 WP_012541132.1 antitoxin Antitoxin
D4N21_RS11055 2240079..2240411 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D4N21_RS11065 2240669..2242105 - 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
D4N21_RS11075 2242543..2243997 - 1455 WP_008804170.1 AMP nucleosidase -
D4N21_RS11080 2244128..2244373 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T111683 WP_008804165.1 NZ_CP032354:2240079-2240411 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T111683 NZ_CP032354:2240079-2240411 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9388.85 Da        Isoelectric Point: 4.4915

>AT111683 WP_012541132.1 NZ_CP032354:2239821-2240078 [Klebsiella variicola]
VYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT111683 NZ_CP032354:2239821-2240078 [Klebsiella variicola]
GTGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References