Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1245225..1245882 Replicon chromosome
Accession NZ_CP032354
Organism Klebsiella variicola strain 15WZ-82

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag D4N21_RS06220 Protein ID WP_002916310.1
Coordinates 1245472..1245882 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag D4N21_RS06215 Protein ID WP_002916312.1
Coordinates 1245225..1245491 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N21_RS06190 1240322..1241755 - 1434 WP_012967244.1 6-phospho-beta-glucosidase BglA -
D4N21_RS06195 1241875..1242603 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
D4N21_RS06200 1242654..1242965 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
D4N21_RS06205 1243129..1243788 + 660 WP_008806429.1 hemolysin III family protein -
D4N21_RS06210 1243996..1244979 - 984 WP_032733797.1 tRNA-modifying protein YgfZ -
D4N21_RS06215 1245225..1245491 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
D4N21_RS06220 1245472..1245882 + 411 WP_002916310.1 protein YgfX Toxin
D4N21_RS06225 1245889..1246410 - 522 WP_008806427.1 flavodoxin FldB -
D4N21_RS06230 1246511..1247407 + 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
D4N21_RS06235 1247430..1248143 + 714 WP_064166672.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D4N21_RS06240 1248149..1249882 + 1734 WP_064166671.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T111682 WP_002916310.1 NZ_CP032354:1245472-1245882 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T111682 NZ_CP032354:1245472-1245882 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT111682 WP_002916312.1 NZ_CP032354:1245225-1245491 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT111682 NZ_CP032354:1245225-1245491 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References