Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 764579..765165 Replicon chromosome
Accession NZ_CP032354
Organism Klebsiella variicola strain 15WZ-82

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag D4N21_RS03655 Protein ID WP_002920800.1
Coordinates 764797..765165 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A1F2M0C2
Locus tag D4N21_RS03650 Protein ID WP_032731361.1
Coordinates 764579..764800 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N21_RS03630 760736..761662 + 927 WP_101837392.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
D4N21_RS03635 761659..762936 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
D4N21_RS03640 762933..763700 + 768 WP_032731362.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
D4N21_RS03645 763702..764415 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
D4N21_RS03650 764579..764800 + 222 WP_032731361.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D4N21_RS03655 764797..765165 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
D4N21_RS03660 765457..766773 + 1317 WP_086892791.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
D4N21_RS03665 766880..767767 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
D4N21_RS03670 767764..768609 + 846 WP_008806976.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
D4N21_RS03675 768611..769681 + 1071 WP_008806977.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 761659..770418 8759


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T111680 WP_002920800.1 NZ_CP032354:764797-765165 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T111680 NZ_CP032354:764797-765165 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8165.18 Da        Isoelectric Point: 4.7769

>AT111680 WP_032731361.1 NZ_CP032354:764579-764800 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT111680 NZ_CP032354:764579-764800 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTATACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAATTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M0C2

References