Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 347603..348228 Replicon chromosome
Accession NZ_CP032354
Organism Klebsiella variicola strain 15WZ-82

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag D4N21_RS01700 Protein ID WP_008807903.1
Coordinates 347603..347986 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag D4N21_RS01705 Protein ID WP_004150355.1
Coordinates 347986..348228 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N21_RS01685 344969..345871 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
D4N21_RS01690 345868..346503 + 636 WP_128205686.1 formate dehydrogenase cytochrome b556 subunit -
D4N21_RS01695 346500..347429 + 930 WP_055323209.1 formate dehydrogenase accessory protein FdhE -
D4N21_RS01700 347603..347986 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
D4N21_RS01705 347986..348228 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
D4N21_RS01710 348433..349350 + 918 WP_128205688.1 alpha/beta hydrolase -
D4N21_RS01715 349365..350306 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
D4N21_RS01720 350351..350788 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
D4N21_RS01725 350785..351645 - 861 WP_008807907.1 virulence factor BrkB family protein -
D4N21_RS01730 351639..352238 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T111679 WP_008807903.1 NZ_CP032354:c347986-347603 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T111679 NZ_CP032354:c347986-347603 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT111679 WP_004150355.1 NZ_CP032354:c348228-347986 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT111679 NZ_CP032354:c348228-347986 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References