Detailed information of TA system TA111676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2941851..2943127
• Replicon: chromosome
• Accession: NZ_CP032353
• Organism: Pseudomonas protegens strain pf5-k2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q4KD87
• Locus tag: D4N37_RS13845
• Protein ID: WP_011060985.1
• Coordinates: 2942528..2943127 (+)
• Length: 200 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D4N37_RS13840
• Protein ID: WP_011060984.1
• Coordinates: 2941851..2942408 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N37_RS13810	2937055..2937705	+	651	WP_011060978.1	DUF2239 family protein	-
D4N37_RS13815	2937793..2938413	+	621	WP_011060979.1	LysE family translocator	-
D4N37_RS13820	2938459..2939046	-	588	WP_011060980.1	inclusion body family protein	-
D4N37_RS13825	2939506..2940693	+	1188	WP_011060981.1	hypothetical protein	-
D4N37_RS13830	2940872..2941282	-	411	WP_041774583.1	hypothetical protein	-
D4N37_RS13835	2941320..2941661	-	342	WP_011060983.1	low molecular weight protein tyrosine phosphatase family protein	-
D4N37_RS13840	2941851..2942408	+	558	WP_011060984.1	helix-turn-helix domain-containing protein	Antitoxin
D4N37_RS13845	2942528..2943127	+	600	WP_011060985.1	HD domain-containing protein	Toxin
D4N37_RS13850	2943341..2945521	+	2181	WP_011060986.1	catalase/peroxidase HPI	-
D4N37_RS13855	2945583..2946269	-	687	WP_011060987.1	hypothetical protein	-
D4N37_RS13860	2946229..2946849	-	621	WP_011060988.1	hypothetical protein	-
D4N37_RS13865	2946897..2947325	-	429	WP_011060989.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (8-193)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-48)

Sequences

Toxin

Protein

• Length: 200 a.a.
• Molecular weight: 22413.22 Da
• Isoelectric Point: 5.8382

>T111676 WP_011060985.1 NZ_CP032353:2942528-2943127 [Pseudomonas protegens]
MNSELLSGRLEFLREAEKLKDVLRSAHTSSGRQESTAEHSWRLCLMALLFEDQLGDLDLLRVLKLCIVHDLGEAIHGDIP
AVAQAAHPDKSPQERADLQLLARALDAPAREHLLELWDEYEGAASNEARAVKALDKLETLLQHTQGQNPAGFDYRFNLGY
GRRYTDASPLFQALRQQIDADTQRRLDAQAQAAVQHRLR

Nucleotide

• Length: 600 bp

>T111676 NZ_CP032353:2942528-2943127 [Pseudomonas protegens]
ATGAACAGTGAACTTCTCAGTGGCCGCCTTGAATTCCTCCGTGAAGCGGAAAAACTCAAGGACGTGCTGCGCAGTGCCCA
CACCTCCAGCGGCCGCCAGGAAAGCACCGCCGAGCACAGCTGGCGCCTGTGCCTGATGGCGCTGCTGTTCGAAGACCAAC
TGGGGGATCTGGACCTACTGCGGGTGCTGAAGCTGTGCATCGTTCACGACCTGGGGGAGGCCATTCACGGCGATATCCCG
GCGGTGGCCCAGGCCGCCCACCCGGACAAGAGCCCGCAGGAGCGCGCGGACCTGCAACTGCTGGCCCGAGCCCTGGACGC
CCCGGCCCGCGAGCACCTGCTGGAGCTGTGGGACGAATACGAAGGCGCCGCCAGCAACGAGGCGCGCGCGGTCAAGGCCC
TGGACAAGCTGGAAACCCTGCTGCAACACACCCAGGGCCAGAACCCGGCGGGTTTCGACTACCGCTTCAACCTCGGCTAC
GGCCGCCGCTACACCGATGCCAGCCCGCTGTTCCAGGCCCTGCGCCAGCAGATCGATGCCGACACCCAGCGCCGCCTGGA
CGCCCAGGCTCAGGCCGCGGTTCAGCATCGGCTGCGCTAA

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20446.44 Da
• Isoelectric Point: 7.4189

>AT111676 WP_011060984.1 NZ_CP032353:2941851-2942408 [Pseudomonas protegens]
MSVALFTSPHLGLALRRWRLLHRVKQQHAAELFGVAQSSISRWENGRQAMEPAERARLEQLLAANLSAAADQALARLVHD
SPRAVHLVCDLTHRLLACSVTRAGQFGVPLSELLGQSLWGFCTEEIVRKEAALDDLGWREVLAPPSLEFFSGHNDSAIVP
IAPSQCRWTRLNLSDGNAVRLVETL

Nucleotide

• Length: 558 bp

>AT111676 NZ_CP032353:2941851-2942408 [Pseudomonas protegens]
ATGTCCGTAGCTCTGTTCACCTCACCCCATCTGGGCCTGGCCCTCAGGCGCTGGCGCCTGTTGCACCGGGTCAAGCAGCA
GCACGCCGCCGAGTTGTTCGGTGTGGCGCAGTCGAGCATTTCCCGCTGGGAAAACGGCCGGCAAGCCATGGAACCGGCGG
AACGCGCGCGCTTGGAGCAACTGCTGGCGGCCAACCTCAGCGCCGCCGCCGACCAGGCCCTGGCGCGGCTGGTGCATGAC
AGCCCGCGCGCCGTGCACCTGGTGTGCGACCTGACCCACCGCCTGCTGGCCTGTTCCGTGACCCGCGCCGGGCAGTTCGG
CGTGCCCCTGAGCGAGCTGCTGGGGCAATCCCTGTGGGGCTTTTGCACCGAGGAAATCGTGCGCAAGGAAGCCGCCCTGG
ATGACCTGGGCTGGCGCGAAGTACTGGCCCCGCCGTCCCTGGAATTCTTCAGCGGCCACAACGATTCGGCCATCGTGCCC
ATCGCCCCCAGCCAGTGCCGCTGGACCCGCCTCAACCTCTCCGACGGCAACGCCGTGCGGCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: Q4KD87