Detailed information of TA system TA111675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 737739..738335
• Replicon: chromosome
• Accession: NZ_CP032353
• Organism: Pseudomonas protegens strain pf5-k2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q4KIY8
• Locus tag: D4N37_RS03285
• Protein ID: WP_011059021.1
• Coordinates: 738033..738335 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4KIY9
• Locus tag: D4N37_RS03280
• Protein ID: WP_011059020.1
• Coordinates: 737739..738029 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N37_RS03260	734009..736141	-	2133	WP_011059016.1	TonB-dependent copper receptor	-
D4N37_RS03265	736230..736640	-	411	WP_011059017.1	DUF2946 domain-containing protein	-
D4N37_RS03270	736696..737175	-	480	WP_026020078.1	copper chaperone PCu(A)C	-
D4N37_RS03275	737240..737638	-	399	WP_011059019.1	DUF2946 domain-containing protein	-
D4N37_RS03280	737739..738029	-	291	WP_011059020.1	putative addiction module antidote protein	Antitoxin
D4N37_RS03285	738033..738335	-	303	WP_011059021.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D4N37_RS03290	738519..739247	-	729	WP_011059022.1	cobalt-precorrin-6A reductase	-
D4N37_RS03295	739244..740341	-	1098	WP_011059023.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
D4N37_RS03300	740435..741649	-	1215	WP_011059024.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
D4N37_RS03305	741788..743101	+	1314	WP_041774047.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11499.32 Da
• Isoelectric Point: 10.4481

>T111675 WP_011059021.1 NZ_CP032353:c738335-738033 [Pseudomonas protegens]
MFVFEQTPEFSRWLLNLKDPQGKTRVLARIRAAQLGRFGDCEPVGEGVHEIRIHRGPGYRVYFSRHGKMIYLLLIGGDKA
TQKRDIQRARQLACDLKSEG

Nucleotide

• Length: 303 bp

>T111675 NZ_CP032353:c738335-738033 [Pseudomonas protegens]
ATGTTCGTTTTCGAGCAGACTCCTGAGTTCAGCCGCTGGCTGCTGAACCTGAAGGACCCGCAAGGGAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCACAGCTGGGCCGTTTTGGCGACTGCGAACCTGTGGGCGAAGGCGTCCACGAGATACGCATCC
ACCGCGGCCCGGGTTACCGGGTGTACTTCTCGCGCCACGGCAAAATGATCTACCTGCTGTTGATCGGCGGCGACAAGGCC
ACCCAGAAACGCGATATCCAACGGGCCAGGCAACTGGCCTGCGACCTCAAGAGCGAGGGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10423.94 Da
• Isoelectric Point: 5.0939

>AT111675 WP_011059020.1 NZ_CP032353:c738029-737739 [Pseudomonas protegens]
MRTPVTRFDAAEYLKTPEDMVAYLDACFEEDTGDGVLIRAALNDIARARGMTQVARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKINVSNA

Nucleotide

• Length: 291 bp

>AT111675 NZ_CP032353:c738029-737739 [Pseudomonas protegens]
ATGCGCACCCCAGTGACCCGCTTCGACGCGGCGGAGTACCTCAAGACCCCTGAAGACATGGTCGCCTACCTCGACGCCTG
CTTCGAGGAAGACACCGGCGACGGCGTGCTGATCCGCGCCGCACTCAACGACATCGCCCGTGCCCGCGGCATGACCCAGG
TCGCCCGTGACACCGGCCTGGGCCGCGAAAGCCTGTACAAGGCCCTCGGCAGCCAGGGCAACCCGGAGTTCGCGACTATC
ATCAAGGTGATGAAGGCCCTGGGGTTGAAGATCAACGTCAGCAACGCCTGA

Structures

Toxin

• AlphaFold DB: Q4KIY8

Antitoxin

• AlphaFold DB: Q4KIY9