Detailed information of TA system TA111664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3433457..3434232
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: D3VFY6
• Locus tag: D3790_RS15665
• Protein ID: WP_013185762.1
• Coordinates: 3433457..3433957 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: N1NS87
• Locus tag: D3790_RS15670
• Protein ID: WP_010846171.1
• Coordinates: 3433954..3434232 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS15630	3429223..3429777	+	555	WP_038219384.1	cytochrome b	-
D3790_RS15635	3429798..3430379	+	582	WP_010846163.1	YceI family protein	-
D3790_RS15640	3430537..3430850	+	314	Protein_2979	type II toxin-antitoxin system RelE/ParE family toxin	-
D3790_RS15645	3430847..3431128	+	282	WP_010846165.1	helix-turn-helix transcriptional regulator	-
D3790_RS15650	3431433..3431696	+	264	WP_038219381.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
D3790_RS15655	3431696..3432085	+	390	WP_013185764.1	type II toxin-antitoxin system VapC family toxin	-
D3790_RS15660	3432428..3433369	-	942	WP_013185763.1	phenylacetic acid degradation operon negative regulatory protein PaaX	-
D3790_RS15665	3433457..3433957	-	501	WP_013185762.1	GNAT family N-acetyltransferase	Toxin
D3790_RS15670	3433954..3434232	-	279	WP_010846171.1	DUF1778 domain-containing protein	Antitoxin
D3790_RS15675	3434370..3435713	-	1344	WP_041977333.1	phenylacetate--CoA ligase	-
D3790_RS15680	3435799..3437013	-	1215	WP_038219377.1	3-oxoadipyl-CoA thiolase	-
D3790_RS15685	3437010..3437561	-	552	WP_038219375.1	hydroxyphenylacetyl-CoA thioesterase PaaI	-
D3790_RS15690	3437554..3439182	-	1629	WP_013185758.1	3-hydroxyacyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-83)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18238.10 Da
• Isoelectric Point: 9.0299

>T111664 WP_013185762.1 NZ_CP032329:c3433957-3433457 [Xenorhabdus nematophila]
MILSAPELLSEHHYREAFNSGVTTLDQWLKNRALKNNLTGASKTYVVCSENRVLAYYSLAASAIVTDSTPGHFRRNMPNP
IPVVVLGRLAVDRSLQRKGIGRALVQDAALRIIQAADLIGVRGMIIHALSDEVKSFYETIGFEPSPLDPMLLMATLADLK
RSCTNS

Nucleotide

• Length: 501 bp

>T111664 NZ_CP032329:c3433957-3433457 [Xenorhabdus nematophila]
ATGATTCTTTCGGCACCGGAATTGCTGTCTGAACACCATTATCGTGAAGCGTTTAATTCAGGGGTTACCACCCTAGACCA
GTGGCTCAAAAATCGTGCACTAAAAAATAACTTAACTGGCGCATCAAAAACCTACGTGGTTTGTAGTGAAAATCGAGTAC
TGGCTTATTATTCTCTGGCCGCTAGCGCGATTGTGACTGATTCGACACCCGGCCATTTTAGGCGGAATATGCCTAACCCA
ATCCCTGTCGTCGTTTTAGGCCGATTGGCTGTAGATAGGAGTCTGCAACGAAAAGGCATAGGCCGTGCGTTAGTACAAGA
TGCCGCGTTGAGAATAATCCAAGCTGCTGATTTAATTGGTGTCCGAGGTATGATTATTCATGCGCTTTCAGATGAAGTAA
AATCTTTTTATGAAACCATCGGGTTTGAACCTTCTCCTCTCGATCCCATGTTACTGATGGCTACACTCGCAGATTTAAAA
AGATCCTGTACGAATAGTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10587.22 Da
• Isoelectric Point: 6.3606

>AT111664 WP_010846171.1 NZ_CP032329:c3434232-3433954 [Xenorhabdus nematophila]
MQTPKRDTLSIRIKPETRNLIDKAAAIQGKNRTDFVLEAAQRMAEETLLEQAIMTASPEAYAKFLDRLDMPPQPNKQLRE
TMQMETPWGKEL

Nucleotide

• Length: 279 bp

>AT111664 NZ_CP032329:c3434232-3433954 [Xenorhabdus nematophila]
ATGCAAACGCCAAAGCGAGATACTTTGAGTATTCGTATCAAACCAGAAACTCGGAATTTGATTGATAAAGCAGCCGCCAT
TCAGGGTAAAAACCGCACAGATTTTGTATTGGAAGCGGCACAACGCATGGCCGAAGAAACCTTGCTTGAACAAGCAATTA
TGACGGCCAGTCCAGAAGCATATGCCAAATTTTTGGATCGTTTAGATATGCCTCCACAACCGAATAAGCAATTGCGAGAA
ACCATGCAGATGGAAACGCCTTGGGGCAAGGAATTATGA

Structures

Toxin

• AlphaFold DB: D3VFY6

Antitoxin

• AlphaFold DB: N1NS87