Detailed information of TA system TA111660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3322796..3323440
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D3790_RS15085
• Protein ID: WP_038220160.1
• Coordinates: 3322796..3322969 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N1NK82
• Locus tag: D3790_RS15090
• Protein ID: WP_010847564.1
• Coordinates: 3323024..3323440 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS15065	3319124..3320506	-	1383	WP_013183087.1	envelope stress sensor histidine kinase CpxA	-
D3790_RS15070	3320503..3321195	-	693	WP_013183086.1	envelope stress response regulator transcription factor CpxR	-
D3790_RS15075	3321348..3321848	+	501	WP_010847568.1	Spy/CpxP family protein refolding chaperone	-
D3790_RS15080	3322221..3322664	+	444	WP_010847567.1	hypothetical protein	-
D3790_RS15085	3322796..3322969	+	174	WP_038220160.1	type II toxin-antitoxin system HicA family toxin	Toxin
D3790_RS15090	3323024..3323440	+	417	WP_010847564.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D3790_RS15095	3323548..3324796	+	1249	Protein_2881	IS3 family transposase	-
D3790_RS15105	3325083..3325379	-	297	WP_010847563.1	helix-turn-helix domain-containing protein	-
D3790_RS15110	3325425..3325733	-	309	WP_010847562.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D3790_RS15120	3326079..3326978	+	900	WP_010847560.1	CDF family cation-efflux transporter FieF	-
D3790_RS15125	3327212..3328189	+	978	WP_013183083.1	6-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6680.83 Da
• Isoelectric Point: 11.4032

>T111660 WP_038220160.1 NZ_CP032329:3322796-3322969 [Xenorhabdus nematophila]
VKQSEFRRWLEAQGVEVSNGTNHLKLKYKGRRSIMPRHPSQEIKEPLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T111660 NZ_CP032329:3322796-3322969 [Xenorhabdus nematophila]
GTGAAGCAAAGCGAGTTTAGACGGTGGCTGGAAGCTCAAGGTGTTGAAGTTTCAAACGGGACAAACCATCTGAAACTGAA
ATATAAGGGGAGACGAAGCATTATGCCGAGACACCCCAGCCAAGAGATAAAAGAACCACTGAGGAAAGCAATTCTTAAGC
AATTAGGATTGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15441.59 Da
• Isoelectric Point: 4.2220

>AT111660 WP_010847564.1 NZ_CP032329:3323024-3323440 [Xenorhabdus nematophila]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGETREEALEMALDALITSFEFYFEDNEKIPLPSPIGQDDDYVDVPLSITSKV
LMLNAFIDSKLTQTELASRMGVKKQEVTRIFDLRHSTKIDTVGKAAAVIGHQLTLSMQ

Nucleotide

• Length: 417 bp

>AT111660 NZ_CP032329:3323024-3323440 [Xenorhabdus nematophila]
ATGCGATATCCAGTAACACTAGAGCCAGTGGAAGAAGGCGGTTATTTCGTCTCGTTCCCGGATATCCCCGAAGCTTTAAC
TCAAGGGGAGACACGGGAAGAAGCGCTAGAGATGGCACTTGATGCGCTAATAACGTCTTTCGAATTTTACTTCGAAGATA
ATGAGAAAATTCCGCTTCCTAGCCCTATAGGACAAGATGACGATTATGTCGATGTGCCCTTAAGCATAACCTCTAAGGTG
CTTATGCTTAATGCGTTTATTGATTCTAAATTAACGCAAACTGAACTGGCCAGCCGTATGGGAGTGAAGAAACAGGAAGT
CACGCGCATCTTCGATTTACGGCATTCAACTAAGATAGATACAGTGGGTAAGGCGGCAGCGGTTATCGGGCACCAGTTAA
CATTGTCAATGCAGTAA

Structures

Antitoxin

• AlphaFold DB: N1NK82