Detailed information of TA system TA111656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3033233..3033819
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D3790_RS13515
• Protein ID: WP_038218882.1
• Coordinates: 3033233..3033562 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: N1NFW9
• Locus tag: D3790_RS13520
• Protein ID: WP_010845238.1
• Coordinates: 3033562..3033819 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS13490	3028959..3029606	+	648	WP_038218884.1	peptide-methionine (S)-S-oxide reductase MsrA	-
D3790_RS13495	3029692..3031023	+	1332	WP_010845233.1	hemolysin family protein	-
D3790_RS13500	3031138..3031344	-	207	WP_010845234.1	DUF1107 domain-containing protein	-
D3790_RS13505	3031671..3032228	+	558	WP_010845235.1	YtfJ family protein	-
D3790_RS13510	3032320..3033063	-	744	WP_010845236.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
D3790_RS13515	3033233..3033562	-	330	WP_038218882.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D3790_RS13520	3033562..3033819	-	258	WP_010845238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D3790_RS13525	3034056..3034676	-	621	WP_010845240.1	peptidylprolyl isomerase	-
D3790_RS13530	3034823..3035641	-	819	WP_038218880.1	DUF3037 domain-containing protein	-
D3790_RS13540	3035945..3036313	-	369	WP_010845243.1	hypothetical protein	-
D3790_RS13545	3036538..3036990	-	453	WP_010845244.1	50S ribosomal protein L9	-
D3790_RS13550	3037029..3037256	-	228	WP_010845245.1	30S ribosomal protein S18	-
D3790_RS13555	3037261..3037578	-	318	WP_010845246.1	primosomal replication protein N	-
D3790_RS13560	3037584..3037982	-	399	WP_010845247.1	30S ribosomal protein S6	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• SpoVT-AbrB domain (13-47)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12270.43 Da
• Isoelectric Point: 9.5936

>T111656 WP_038218882.1 NZ_CP032329:c3033562-3033233 [Xenorhabdus nematophila]
MYIPDKGDIISLNSDPSAGKEIMKRRPAFVISRKMFNEHTGFAVVVPITSTARGMKLEVILPEEVSVQGSILIHQVKSLD
FSERQVKFIEKAPQYIIDRVTELTKAIIL

Nucleotide

• Length: 330 bp

>T111656 NZ_CP032329:c3033562-3033233 [Xenorhabdus nematophila]
ATGTATATACCAGACAAAGGTGACATTATTTCATTAAACTCTGATCCGAGTGCAGGCAAGGAAATCATGAAACGTCGTCC
TGCATTCGTTATATCTCGTAAAATGTTTAATGAGCACACTGGATTTGCTGTTGTAGTTCCAATCACGAGTACAGCGAGAG
GAATGAAGTTAGAAGTTATCTTACCCGAAGAAGTATCTGTTCAAGGCTCAATTCTCATTCATCAGGTAAAATCCTTAGAT
TTTTCGGAGCGTCAGGTCAAATTTATTGAGAAAGCGCCTCAATATATTATTGATAGAGTGACTGAGCTAACTAAGGCCAT
CATCTTATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9283.71 Da
• Isoelectric Point: 4.5959

>AT111656 WP_010845238.1 NZ_CP032329:c3033819-3033562 [Xenorhabdus nematophila]
MTAVVIRQSGGANIVSIPKAIVKTLNLHTGSKLELSIQDNKIVLTPVEEELTLESLLAGSPKECFRVTDEDREWLEANPV
GKEVI

Nucleotide

• Length: 258 bp

>AT111656 NZ_CP032329:c3033819-3033562 [Xenorhabdus nematophila]
ATGACTGCTGTTGTCATTCGCCAATCTGGCGGTGCTAACATTGTTAGCATTCCAAAGGCCATAGTGAAAACACTTAATCT
GCATACTGGCTCAAAGCTAGAGCTATCTATTCAGGATAACAAAATTGTACTGACTCCAGTCGAAGAAGAGTTGACGCTGG
AATCGCTGTTAGCAGGAAGCCCTAAAGAATGTTTCAGAGTCACAGATGAGGATAGAGAGTGGCTTGAGGCTAATCCTGTT
GGTAAGGAAGTGATTTGA

Structures

Antitoxin

• AlphaFold DB: N1NFW9