Detailed information of TA system TA111654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2593463..2594081
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: N1NUC1
• Locus tag: D3790_RS11400
• Protein ID: WP_010845060.1
• Coordinates: 2593463..2593666 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: N1NQW2
• Locus tag: D3790_RS11405
• Protein ID: WP_010845061.1
• Coordinates: 2593713..2594081 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS11380	2589970..2590308	+	339	WP_010845056.1	P-II family nitrogen regulator	-
D3790_RS11385	2590323..2591606	+	1284	WP_038220148.1	ammonium transporter AmtB	-
D3790_RS11390	2591781..2592635	-	855	WP_010845058.1	acyl-CoA thioesterase II	-
D3790_RS11400	2593463..2593666	-	204	WP_010845060.1	hemolysin expression modulator Hha	Toxin
D3790_RS11405	2593713..2594081	-	369	WP_010845061.1	Hha toxicity modulator TomB	Antitoxin
D3790_RS11410	2594597..2595623	+	1027	Protein_2194	IS630 family transposase	-
D3790_RS11415	2595850..2597098	+	1249	Protein_2195	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8077.34 Da
• Isoelectric Point: 6.9770

>T111654 WP_010845060.1 NZ_CP032329:c2593666-2593463 [Xenorhabdus nematophila]
MTKTDYLMRLRKCTSIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 204 bp

>T111654 NZ_CP032329:c2593666-2593463 [Xenorhabdus nematophila]
ATGACTAAAACTGACTATCTGATGCGTTTGAGAAAATGCACTTCTATTGAAACACTGGAACGTGTAATTGAAAAAAATAA
ATATGAACTTTCGGATGATGAACTTGAATTGTTCTACTCCGCTGCTGACCACCGTTTAGCCGAACTTACCATGAATAAAC
TCTATGATAAAATCCCATCATCTGTTTGGAAATTCGTCCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14490.38 Da
• Isoelectric Point: 5.1920

>AT111654 WP_010845061.1 NZ_CP032329:c2594081-2593713 [Xenorhabdus nematophila]
MDEYSPKRYDIAELKYLCEKLIHEALSVLNRADSHWIHDLTSEKSLKLNELIEHIAAFVWNFKIKYTDHYDLSTLIDEYL
DETYNLFGSDKISFLELTKWQRTNEHLTNILLHDLNASLSKT

Nucleotide

• Length: 369 bp

>AT111654 NZ_CP032329:c2594081-2593713 [Xenorhabdus nematophila]
ATGGATGAATATTCACCAAAAAGATATGATATCGCTGAATTAAAATACTTGTGTGAAAAATTAATTCATGAGGCACTTTC
AGTCCTGAATCGCGCTGATAGTCACTGGATTCACGATCTGACTTCCGAGAAAAGCTTAAAATTAAACGAATTGATCGAAC
ACATTGCAGCTTTTGTCTGGAACTTTAAAATTAAATACACTGATCACTACGATCTCAGCACCTTAATTGATGAGTATCTG
GATGAAACATATAATTTGTTTGGAAGCGATAAGATAAGCTTTCTTGAACTTACTAAATGGCAAAGAACAAACGAACACTT
AACTAACATTCTGCTACATGATTTGAATGCTTCTTTAAGTAAAACCTAA

Structures

Toxin

• AlphaFold DB: N1NUC1

Antitoxin

• AlphaFold DB: N1NQW2