Detailed information of TA system TA111650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 112955..113537
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: D3VJ77
• Locus tag: D3790_RS00570
• Protein ID: WP_013184681.1
• Coordinates: 112955..113242 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D3790_RS00575
• Protein ID: WP_010846808.1
• Coordinates: 113253..113537 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS00545	109938..110810	+	873	WP_081480167.1	IS630 family transposase	-
D3790_RS00550	111140..111565	-	426	WP_012987825.1	type II toxin-antitoxin system VapC family toxin	-
D3790_RS00555	111565..111819	-	255	WP_010846804.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D3790_RS00560	112044..112373	+	330	WP_010846805.1	type I toxin-antitoxin system SymE family toxin	-
D3790_RS00565	112514..112852	+	339	WP_010846806.1	SymE family type I addiction module toxin	-
D3790_RS00570	112955..113242	+	288	WP_013184681.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D3790_RS00575	113253..113537	+	285	WP_010846808.1	putative addiction module antidote protein	Antitoxin
D3790_RS00580	113620..114003	-	384	WP_041976221.1	hypothetical protein	-
D3790_RS00585	114124..114333	-	210	WP_013184683.1	hypothetical protein	-
D3790_RS00590	114456..115487	-	1032	WP_038221118.1	IS630 family transposase	-
D3790_RS00595	115569..115766	+	198	WP_081990858.1	replication initiation protein	-
D3790_RS00600	115816..116430	-	615	WP_010849037.1	DUF4158 domain-containing protein	-
D3790_RS00605	116626..117243	+	618	WP_010849038.1	recombinase family protein	-
D3790_RS00610	117319..117573	+	255	WP_052145619.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	83165..173875	90710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-92)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10773.43 Da
• Isoelectric Point: 10.0371

>T111650 WP_013184681.1 NZ_CP032329:112955-113242 [Xenorhabdus nematophila]
MITIERTQNFEKWLKSLKDRIAKAKILIRVERMGEGNFGDVESVGGGVSELRIHYGQGYRVYFTNKNNEIILLLCGGDKS
SQQADIKKAKQLAKE

Nucleotide

• Length: 288 bp

>T111650 NZ_CP032329:112955-113242 [Xenorhabdus nematophila]
ATGATAACCATTGAAAGGACACAAAATTTTGAAAAATGGTTAAAATCCTTAAAAGACCGAATAGCAAAAGCCAAAATCTT
AATCAGAGTTGAAAGAATGGGAGAGGGCAATTTTGGTGATGTTGAATCGGTGGGAGGTGGAGTTTCGGAACTCAGAATAC
ATTACGGGCAAGGCTATCGTGTCTACTTTACTAACAAAAATAATGAAATCATATTATTACTCTGTGGCGGGGATAAAAGC
AGCCAGCAGGCAGATATAAAGAAAGCCAAACAACTCGCGAAAGAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10443.90 Da
• Isoelectric Point: 9.3655

>AT111650 WP_010846808.1 NZ_CP032329:113253-113537 [Xenorhabdus nematophila]
MKSAPKKFDVVEFLETEEDIQAYLNAAIEENDTKYLFKALGNIARRKNISQLSKESGISREGIYKALSGEGNPSFNTVYK
ISKALGLKLHFTGA

Nucleotide

• Length: 285 bp

>AT111650 NZ_CP032329:113253-113537 [Xenorhabdus nematophila]
ATGAAATCAGCACCAAAAAAATTTGATGTTGTGGAGTTTTTAGAAACAGAAGAAGATATTCAGGCATACTTAAATGCCGC
TATCGAAGAAAACGATACAAAATATTTATTTAAAGCATTAGGTAATATCGCCAGAAGAAAAAATATCAGCCAACTCTCGA
AAGAATCAGGAATAAGCCGAGAAGGGATATATAAGGCGTTATCCGGTGAAGGCAATCCATCGTTTAATACGGTATACAAA
ATATCGAAAGCACTGGGTTTGAAACTGCATTTTACGGGGGCTTAA

Structures

Toxin

• AlphaFold DB: D3VJ77