Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 511393..512029 Replicon chromosome
Accession NZ_CP032310
Organism Bacillus subtilis strain WB800N

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D3Z87_RS02610 Protein ID WP_003156187.1
Coordinates 511679..512029 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag D3Z87_RS02605 Protein ID WP_003225183.1
Coordinates 511393..511674 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3Z87_RS02585 507752..508351 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
D3Z87_RS02590 508446..508811 + 366 WP_003234281.1 holo-ACP synthase -
D3Z87_RS02595 508977..509993 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
D3Z87_RS02600 510108..511277 + 1170 WP_120322737.1 alanine racemase -
D3Z87_RS02605 511393..511674 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D3Z87_RS02610 511679..512029 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D3Z87_RS02615 512144..512968 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
D3Z87_RS02620 512973..513338 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
D3Z87_RS02625 513342..513743 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
D3Z87_RS02630 513755..514762 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
D3Z87_RS02635 514824..515153 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
D3Z87_RS02640 515150..515632 + 483 WP_003234299.1 anti-sigma B factor RsbW -
D3Z87_RS02645 515598..516386 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
D3Z87_RS02650 516386..516985 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T111614 WP_003156187.1 NZ_CP032310:511679-512029 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T111614 NZ_CP032310:511679-512029 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT111614 WP_003225183.1 NZ_CP032310:511393-511674 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT111614 NZ_CP032310:511393-511674 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References