Detailed information of TA system TA111613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1172519..1173012
• Replicon: chromosome
• Accession: NZ_CP032308
• Organism: Enterococcus faecium strain HY07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CTI32_RS07465
• Protein ID: WP_002292138.1
• Coordinates: 1172746..1173012 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CTI32_RS07460
• Protein ID: WP_002292139.1
• Coordinates: 1172519..1172749 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI32_RS07420	1168433..1169191	+	759	WP_002342169.1	restriction endonuclease	-
CTI32_RS07430	1169423..1169848	-	426	WP_119364934.1	toxin	-
CTI32_RS07435	1169865..1170122	-	258	WP_002313665.1	hypothetical protein	-
CTI32_RS07440	1170266..1171225	+	960	WP_119364935.1	IS30 family transposase	-
CTI32_RS14835	1171389..1171691	+	303	Protein_1097	hypothetical protein	-
CTI32_RS14840	1171707..1172043	+	337	Protein_1098	hypothetical protein	-
CTI32_RS07460	1172519..1172749	+	231	WP_002292139.1	hypothetical protein	Antitoxin
CTI32_RS07465	1172746..1173012	+	267	WP_002292138.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTI32_RS07470	1173264..1173491	+	228	WP_002313668.1	hypothetical protein	-
CTI32_RS07475	1173507..1174673	+	1167	WP_119364936.1	oxygen-independent coproporphyrinogen III oxidase	-
CTI32_RS07480	1174907..1175950	+	1044	WP_119364937.1	heat-inducible transcriptional repressor HrcA	-
CTI32_RS07485	1176044..1176607	+	564	WP_002292135.1	nucleotide exchange factor GrpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1170266..1171225	959
	-	inside	Integrative and Conjugative Element	-	cpsA / cpsB	1133039..1331260	198221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-72)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10556.21 Da
• Isoelectric Point: 10.3338

>T111613 WP_002292138.1 NZ_CP032308:1172746-1173012 [Enterococcus faecium]
MTYRLEFTPEIQKQLRKMDKHQATLIVRWLYQHIDGIDDPRKFGKGLTANRSGQWRYRIGKYRVLVEIEDNQIVVIAIQV
GHRKNVYD

Nucleotide

• Length: 267 bp

>T111613 NZ_CP032308:1172746-1173012 [Enterococcus faecium]
ATGACCTACCGTTTAGAATTCACGCCTGAAATTCAGAAACAACTTCGTAAAATGGACAAGCATCAAGCGACGCTGATCGT
TCGATGGCTATATCAACATATTGATGGTATTGACGATCCGAGAAAATTCGGAAAAGGATTGACCGCTAATCGTTCAGGAC
AATGGCGTTATCGAATTGGCAAATATCGTGTGTTGGTAGAGATAGAAGATAATCAAATAGTTGTTATCGCTATTCAAGTG
GGACACCGAAAAAATGTCTATGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8883.02 Da
• Isoelectric Point: 4.1943

>AT111613 WP_002292139.1 NZ_CP032308:1172519-1172749 [Enterococcus faecium]
MAMITVRVSDAEKEWLSYMAEFYGITLSELLKTYSMEQLEDEYDRQTAEIAHKHWVESGKTTVSMEEILREFGGLE

Nucleotide

• Length: 231 bp

>AT111613 NZ_CP032308:1172519-1172749 [Enterococcus faecium]
ATGGCTATGATTACGGTTCGTGTGAGTGATGCCGAAAAAGAATGGCTTTCTTATATGGCGGAATTTTATGGGATTACTTT
ATCTGAGCTTCTAAAAACCTATTCGATGGAACAACTAGAAGATGAATACGATCGTCAAACGGCTGAAATTGCCCATAAAC
ATTGGGTAGAAAGTGGAAAAACAACAGTATCCATGGAAGAAATCCTTCGTGAATTTGGAGGGTTGGAATGA