Detailed information of TA system TA111612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/-
• Location: 1169423..1170122
• Replicon: chromosome
• Accession: NZ_CP032308
• Organism: Enterococcus faecium strain HY07

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: CTI32_RS07430
• Protein ID: WP_119364934.1
• Coordinates: 1169423..1169848 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: CTI32_RS07435
• Protein ID: WP_002313665.1
• Coordinates: 1169865..1170122 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI32_RS07400	1165156..1165746	-	591	WP_002308925.1	glutamine amidotransferase	-
CTI32_RS07405	1166471..1167139	+	669	WP_119364933.1	zeta toxin family protein	-
CTI32_RS14760	1167132..1167293	+	162	WP_002342167.1	hypothetical protein	-
CTI32_RS07410	1167503..1167934	+	432	WP_002342168.1	N-6 DNA methylase	-
CTI32_RS07420	1168433..1169191	+	759	WP_002342169.1	restriction endonuclease	-
CTI32_RS07430	1169423..1169848	-	426	WP_119364934.1	toxin	Toxin
CTI32_RS07435	1169865..1170122	-	258	WP_002313665.1	hypothetical protein	Antitoxin
CTI32_RS07440	1170266..1171225	+	960	WP_119364935.1	IS30 family transposase	-
CTI32_RS14835	1171389..1171691	+	303	Protein_1097	hypothetical protein	-
CTI32_RS14840	1171707..1172043	+	337	Protein_1098	hypothetical protein	-
CTI32_RS07460	1172519..1172749	+	231	WP_002292139.1	hypothetical protein	-
CTI32_RS07465	1172746..1173012	+	267	WP_002292138.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CTI32_RS07470	1173264..1173491	+	228	WP_002313668.1	hypothetical protein	-
CTI32_RS07475	1173507..1174673	+	1167	WP_119364936.1	oxygen-independent coproporphyrinogen III oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	cpsA / cpsB	1133039..1331260	198221
	-	flank	IS/Tn	-	-	1170266..1171225	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16705.81 Da
• Isoelectric Point: 4.6731

>T111612 WP_119364934.1 NZ_CP032308:c1169848-1169423 [Enterococcus faecium]
MNVISEGLYFEAADLANELSEEVSYYCSKKMEHVKCYDIENYVNEVEDVEFIDYDFKDNLSKKMLGSITKVHGEIIITTN
QHLMLERKNFTKMHEIIHYYKDVPYLGESHAFSDMILEDTYLPEDYKKTSSKYRCQYPHGE

Nucleotide

• Length: 426 bp

>T111612 NZ_CP032308:c1169848-1169423 [Enterococcus faecium]
TTGAATGTGATTTCTGAAGGACTTTATTTTGAAGCTGCAGACCTTGCAAATGAACTCTCAGAAGAAGTTAGTTATTACTG
TTCAAAGAAAATGGAACATGTAAAATGCTACGACATTGAGAATTATGTGAATGAAGTAGAAGATGTAGAATTCATCGATT
ACGACTTCAAAGATAACTTAAGTAAGAAAATGCTTGGTTCCATTACAAAAGTTCATGGCGAAATCATCATTACCACCAAT
CAACATTTAATGCTTGAACGCAAAAATTTTACAAAAATGCATGAGATCATCCATTATTATAAGGACGTCCCCTATCTTGG
AGAATCTCATGCATTTTCTGATATGATTTTAGAAGATACTTATTTGCCAGAAGACTATAAAAAAACTTCGAGCAAATATA
GGTGCCAGTATCCTCATGGCGAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9913.09 Da
• Isoelectric Point: 4.2290

>AT111612 WP_002313665.1 NZ_CP032308:c1170122-1169865 [Enterococcus faecium]
MLSNYQLVTVIADFFNVSTDFLLNRTSDPTPPKRSEEDQLGDSILSHFRLNTADMEVEDIEELEEELNEYMDFLVQRAKA
KKNKK

Nucleotide

• Length: 258 bp

>AT111612 NZ_CP032308:c1170122-1169865 [Enterococcus faecium]
TTGTTGTCAAATTATCAGCTAGTCACAGTAATAGCAGATTTCTTCAATGTTTCTACAGATTTTCTACTAAATAGAACCTC
TGATCCGACTCCTCCAAAACGTAGCGAAGAAGATCAGCTTGGAGACAGTATTCTTTCTCACTTCAGATTGAATACTGCAG
ATATGGAAGTAGAAGACATTGAAGAATTGGAAGAAGAATTAAATGAATATATGGACTTTTTAGTTCAACGTGCAAAAGCA
AAGAAAAATAAAAAATAG