Detailed information of TA system TA111611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 182885..183486
• Replicon: plasmid unnamed2
• Accession: NZ_CP032307
• Organism: Enterococcus faecium strain HY07

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0V7YM55
• Locus tag: CTI32_RS01040
• Protein ID: WP_002330364.1
• Coordinates: 182885..183229 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6B3Q8V0
• Locus tag: CTI32_RS01045
• Protein ID: WP_002318881.1
• Coordinates: 183223..183486 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI32_RS01000	178244..178555	-	312	WP_104886006.1	hypothetical protein	-
CTI32_RS01005	179449..180054	-	606	Protein_197	recombinase family protein	-
CTI32_RS01015	180635..180799	+	165	WP_025477461.1	class II bacteriocin	-
CTI32_RS01020	180804..181070	+	267	WP_002291092.1	hypothetical protein	-
CTI32_RS01025	181444..181653	+	210	Protein_200	recombinase family protein	-
CTI32_RS01035	182465..182770	-	306	Protein_202	IS982 family transposase	-
CTI32_RS01040	182885..183229	-	345	WP_002330364.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CTI32_RS01045	183223..183486	-	264	WP_002318881.1	PbsX family transcriptional regulator	Antitoxin
CTI32_RS01050	183673..184212	+	540	Protein_205	IS30 family transposase	-
CTI32_RS01060	184634..184876	-	243	WP_025480436.1	hypothetical protein	-
CTI32_RS01065	184758..184997	-	240	Protein_207	IS6 family transposase	-
CTI32_RS01070	185075..185680	-	606	Protein_208	IS30 family transposase	-
CTI32_RS01075	185807..186757	-	951	Protein_209	class 1b ribonucleoside-diphosphate reductase subunit beta	-
CTI32_RS01080	186837..187790	-	954	WP_119364678.1	ribonucleotide-diphosphate reductase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..258852	258852
	-	inside	IScluster/Tn	-	-	181708..185680	3972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13024.11 Da
• Isoelectric Point: 8.8971

>T111611 WP_002330364.1 NZ_CP032307:c183229-182885 [Enterococcus faecium]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Nucleotide

• Length: 345 bp

>T111611 NZ_CP032307:c183229-182885 [Enterococcus faecium]
ATGGTAGATGTTCCAAGACAAGGTGATATTCTTTTATTGAACACAGCTCCCAGATCTGGCCATGAACAGACTGGAAGAAG
ACCTTATATTGTTCTTAGTCACGATATTATTGCAGATACTAGTAATGTGGTTATTGTTGCACCGATAACTACAACTAGTA
GAAATTACCCACTATACATTGAAATAACAGAAAATCATAAAATGAAAACACGCGGAAAAGTTATGTTAGATCAAATCACA
ACAATTGATTATGAAGCCAGGAACTGCAAATTTCTAGAAAAAGCTTCTGAAAAATTAATAGAAGAATTATTAACAAAAAT
ACGAGCTGTATTTCAAAAAGTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10071.69 Da
• Isoelectric Point: 5.2300

>AT111611 WP_002318881.1 NZ_CP032307:c183486-183223 [Enterococcus faecium]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Nucleotide

• Length: 264 bp

>AT111611 NZ_CP032307:c183486-183223 [Enterococcus faecium]
ATGGAAATGATACCAATCAAAAAATGGGGAAATAGCAACGGACTTAGATTGCCAAAAAATTTAATGGAATTTCTGGAATT
AAAGACTGATGATCGAGTATCAATCAAAAGCGAAGATGTTAATGGAAAAAGACGATTAATTATCGAACCAGCAATTTCAA
AAGAAGAAGTAACTATTGAAGAGCTTTTCAAAGATTACACAGAGGGCAAAGTAAATATTAAAATTCAGGATTTAGGAAGC
CCAGTAGGGAGAGAACTATGGTAG

Structures

Toxin

• AlphaFold DB: A0A0V7YM55

Antitoxin

• AlphaFold DB: A0A6B3Q8V0