Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 182885..183486 Replicon plasmid unnamed2
Accession NZ_CP032307
Organism Enterococcus faecium strain HY07

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V7YM55
Locus tag CTI32_RS01040 Protein ID WP_002330364.1
Coordinates 182885..183229 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B3Q8V0
Locus tag CTI32_RS01045 Protein ID WP_002318881.1
Coordinates 183223..183486 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI32_RS01000 178244..178555 - 312 WP_104886006.1 hypothetical protein -
CTI32_RS01005 179449..180054 - 606 Protein_197 recombinase family protein -
CTI32_RS01015 180635..180799 + 165 WP_025477461.1 class II bacteriocin -
CTI32_RS01020 180804..181070 + 267 WP_002291092.1 hypothetical protein -
CTI32_RS01025 181444..181653 + 210 Protein_200 recombinase family protein -
CTI32_RS01035 182465..182770 - 306 Protein_202 IS982 family transposase -
CTI32_RS01040 182885..183229 - 345 WP_002330364.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CTI32_RS01045 183223..183486 - 264 WP_002318881.1 PbsX family transcriptional regulator Antitoxin
CTI32_RS01050 183673..184212 + 540 Protein_205 IS30 family transposase -
CTI32_RS01060 184634..184876 - 243 WP_025480436.1 hypothetical protein -
CTI32_RS01065 184758..184997 - 240 Protein_207 IS6 family transposase -
CTI32_RS01070 185075..185680 - 606 Protein_208 IS30 family transposase -
CTI32_RS01075 185807..186757 - 951 Protein_209 class 1b ribonucleoside-diphosphate reductase subunit beta -
CTI32_RS01080 186837..187790 - 954 WP_119364678.1 ribonucleotide-diphosphate reductase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..258852 258852
- inside IScluster/Tn - - 181708..185680 3972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13024.11 Da        Isoelectric Point: 8.8971

>T111611 WP_002330364.1 NZ_CP032307:c183229-182885 [Enterococcus faecium]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Download         Length: 345 bp

>T111611 NZ_CP032307:c183229-182885 [Enterococcus faecium]
ATGGTAGATGTTCCAAGACAAGGTGATATTCTTTTATTGAACACAGCTCCCAGATCTGGCCATGAACAGACTGGAAGAAG
ACCTTATATTGTTCTTAGTCACGATATTATTGCAGATACTAGTAATGTGGTTATTGTTGCACCGATAACTACAACTAGTA
GAAATTACCCACTATACATTGAAATAACAGAAAATCATAAAATGAAAACACGCGGAAAAGTTATGTTAGATCAAATCACA
ACAATTGATTATGAAGCCAGGAACTGCAAATTTCTAGAAAAAGCTTCTGAAAAATTAATAGAAGAATTATTAACAAAAAT
ACGAGCTGTATTTCAAAAAGTATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10071.69 Da        Isoelectric Point: 5.2300

>AT111611 WP_002318881.1 NZ_CP032307:c183486-183223 [Enterococcus faecium]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Download         Length: 264 bp

>AT111611 NZ_CP032307:c183486-183223 [Enterococcus faecium]
ATGGAAATGATACCAATCAAAAAATGGGGAAATAGCAACGGACTTAGATTGCCAAAAAATTTAATGGAATTTCTGGAATT
AAAGACTGATGATCGAGTATCAATCAAAAGCGAAGATGTTAATGGAAAAAGACGATTAATTATCGAACCAGCAATTTCAA
AAGAAGAAGTAACTATTGAAGAGCTTTTCAAAGATTACACAGAGGGCAAAGTAAATATTAAAATTCAGGATTTAGGAAGC
CCAGTAGGGAGAGAACTATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V7YM55

Antitoxin

Source ID Structure
AlphaFold DB A0A6B3Q8V0

References