Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 228789..230608 Replicon plasmid pRAZF7
Accession NZ_CP032297
Organism Rahnella aquatilis strain ZF7

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag D3Z09_RS24260 Protein ID WP_119262329.1
Coordinates 228789..230138 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID H8P0A0
Locus tag D3Z09_RS24265 Protein ID WP_013578106.1
Coordinates 230135..230608 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3Z09_RS24225 223906..224124 - 219 WP_014416654.1 hypothetical protein -
D3Z09_RS24230 224220..224513 - 294 Protein_200 iron dicitrate ABC transporter ATP-binding protein -
D3Z09_RS24235 224678..224896 - 219 WP_013578112.1 YgdI/YgdR family lipoprotein -
D3Z09_RS24240 225299..225649 + 351 WP_112151587.1 hypothetical protein -
D3Z09_RS24245 225762..227087 + 1326 WP_014416651.1 heme anaerobic degradation radical SAM methyltransferase ChuW/HutW -
D3Z09_RS24250 227074..228204 + 1131 WP_119262328.1 heme utilization cystosolic carrier protein HutX -
D3Z09_RS24255 228372..228593 + 222 WP_014416648.1 DUF1471 domain-containing protein -
D3Z09_RS24260 228789..230138 + 1350 WP_119262329.1 type II toxin-antitoxin system HipA family toxin Toxin
D3Z09_RS24265 230135..230608 + 474 WP_013578106.1 helix-turn-helix domain-containing protein Antitoxin
D3Z09_RS24270 230669..230989 - 321 WP_013578105.1 multidrug efflux SMR transporter -
D3Z09_RS24275 231105..231959 - 855 WP_014416647.1 LysR family transcriptional regulator -
D3Z09_RS24280 232047..232760 - 714 WP_112151437.1 DUF554 domain-containing protein -
D3Z09_RS24285 232800..233369 - 570 WP_119262330.1 UbiX family flavin prenyltransferase -
D3Z09_RS24290 233429..234928 - 1500 WP_013578103.1 UbiD family decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-381)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-139)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51233.66 Da        Isoelectric Point: 4.6576

>T111609 WP_119262329.1 NZ_CP032297:228789-230138 [Rahnella aquatilis]
MEFLTLQAFLNNTWVDIANITFPGSEQNNYRLTELNYHSDYAIDNLDRDDNHAASLNHPVSLFFDGDDERGWLKFLDDII
PSGSSRRYWANYLDLEGLTADQQNFILLKHGTLSPVGNLRIKESLPEYNPLAEKLFFTIEDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLLLRCSQEQQIWIDTYQNDTANRDIPYLVKYPRGARTEVDCNILRAEFYFYHELEAMGFDTIPTQNMR
LEEGLTYPSLWLPRFDISFEEEGLIRRFGMESVYSLLRKAPGVTLYHEETLRALIDKISHSHMVTEQGFDFDIPGFVTEW
VQRDLLNIIFGNSDNHGRNTSFLKDERSIRLAPIYDFAPMKADPEGIARTTKWKAPLEIGGEYDYPGIAQSLDDLVPAET
LLSALKNTSHQLSGLKERLEARGIPQQILNMPAISFSFIPEKLRRWGLL

Download         Length: 1350 bp

>T111609 NZ_CP032297:228789-230138 [Rahnella aquatilis]
ATGGAATTTTTAACGTTACAAGCTTTCCTCAATAATACCTGGGTTGATATTGCTAACATCACTTTTCCCGGCAGTGAGCA
GAATAACTATCGTCTGACCGAACTGAATTATCATTCGGATTACGCTATTGATAACCTTGATCGTGACGATAACCATGCCG
CATCGTTGAATCACCCCGTCTCTTTGTTTTTTGATGGTGACGATGAGCGGGGTTGGCTGAAGTTTCTTGATGATATTATT
CCCAGCGGTTCAAGTCGCCGCTATTGGGCAAACTATCTCGATCTCGAAGGACTGACAGCCGATCAGCAAAATTTCATTTT
GCTCAAACATGGCACCTTGTCCCCTGTTGGCAATCTGCGCATAAAAGAATCATTACCGGAATATAATCCGCTGGCTGAAA
AACTTTTCTTCACTATAGAAGATGTTAAAAACCGGGCGGGTGACTTTCTGGACTACGCACAACAACGCGGTGCGGCTGCC
GGTGGCGCGACAGGCGCCGGGGGCGAAGCACCAAAGCTGTTGCTGCGTTGTAGTCAGGAACAGCAAATCTGGATTGATAC
CTACCAGAATGACACTGCCAACCGGGACATTCCCTATTTGGTCAAATATCCCCGTGGCGCAAGAACAGAGGTAGACTGCA
ATATTCTTCGGGCAGAATTCTATTTCTACCATGAGCTTGAAGCCATGGGGTTTGACACGATCCCGACGCAAAACATGCGG
CTGGAAGAAGGGCTGACTTATCCGTCACTCTGGCTTCCGCGTTTTGATATTTCTTTTGAAGAGGAAGGACTTATCAGACG
TTTCGGAATGGAATCAGTCTATTCACTCCTGCGCAAAGCGCCCGGTGTGACGCTGTATCATGAAGAAACCCTCCGTGCAC
TGATCGATAAGATTTCACACAGTCATATGGTGACAGAACAAGGTTTTGATTTTGATATCCCGGGGTTTGTGACTGAATGG
GTACAACGAGATCTGCTCAATATTATTTTTGGTAACAGCGATAATCATGGGCGTAACACCTCTTTCCTGAAAGATGAACG
CAGCATCCGGCTTGCACCAATCTACGATTTTGCGCCGATGAAAGCCGATCCGGAAGGTATTGCCAGAACGACGAAATGGA
AGGCTCCGCTGGAAATCGGCGGTGAATACGATTATCCCGGCATTGCACAATCACTCGATGATCTTGTCCCGGCAGAAACA
TTACTTTCGGCACTTAAAAACACGTCACATCAACTGTCGGGACTGAAAGAGCGGCTGGAAGCCCGTGGCATACCGCAGCA
AATTTTGAACATGCCCGCGATAAGTTTCAGTTTTATTCCTGAAAAGTTACGCCGCTGGGGGTTGTTATGA

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17587.18 Da        Isoelectric Point: 10.9653

>AT111609 WP_013578106.1 NZ_CP032297:230135-230608 [Rahnella aquatilis]
MKEPEIAANSARNTIDKIRAKQNEANHVRRDAGQMLAQEETSSTGKKQAQRRVSNLTKPVSTNDRNQARLDIMRALLLGE
ITQGQALKALRITVLGLKQDAFGKLVTVSRKTLSEVENDKGNYTPDIINKLFRPFGLKVGLVPVSQQIFRTLFERSE

Download         Length: 474 bp

>AT111609 NZ_CP032297:230135-230608 [Rahnella aquatilis]
ATGAAAGAACCAGAGATTGCCGCAAACTCTGCGAGAAATACCATTGATAAAATACGGGCTAAACAAAATGAGGCGAATCA
TGTGCGGCGTGACGCCGGACAAATGCTGGCTCAGGAAGAAACATCTTCCACGGGAAAAAAACAAGCCCAGCGAAGAGTGT
CGAACCTGACGAAACCCGTCAGCACCAATGACCGAAATCAAGCCCGGCTCGACATCATGCGGGCGTTACTTTTGGGTGAA
ATCACGCAGGGACAAGCACTAAAAGCACTGAGGATTACGGTGCTGGGGTTAAAACAAGACGCCTTTGGGAAATTAGTGAC
AGTGTCACGTAAGACGCTATCAGAAGTGGAAAATGATAAGGGAAATTACACGCCTGACATCATCAACAAACTGTTTAGAC
CATTTGGCCTGAAAGTCGGGCTGGTGCCGGTTTCTCAGCAGATATTCAGGACTCTCTTTGAGCGCAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB H8P0A0

References