Detailed information of TA system TA111608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3627609..3628226
• Replicon: chromosome
• Accession: NZ_CP032296
• Organism: Rahnella aquatilis strain ZF7

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H8NYI7
• Locus tag: D3Z09_RS16880
• Protein ID: WP_013576617.1
• Coordinates: 3628023..3628226 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: H8NYI6
• Locus tag: D3Z09_RS16875
• Protein ID: WP_013576616.1
• Coordinates: 3627609..3627977 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3Z09_RS16845	3623599..3623853	+	255	WP_013576610.1	type B 50S ribosomal protein L31	-
D3Z09_RS16850	3623874..3624014	+	141	WP_013576611.1	type B 50S ribosomal protein L36	-
D3Z09_RS16855	3624139..3625017	-	879	WP_013576612.1	metal ABC transporter substrate-binding protein	-
D3Z09_RS16860	3625061..3625918	-	858	WP_013576613.1	metal ABC transporter permease	-
D3Z09_RS16865	3625915..3626643	-	729	WP_173362112.1	ABC transporter ATP-binding protein	-
D3Z09_RS16870	3627026..3627379	+	354	WP_013576615.1	hypothetical protein	-
D3Z09_RS16875	3627609..3627977	+	369	WP_013576616.1	Hha toxicity modulator TomB	Antitoxin
D3Z09_RS16880	3628023..3628226	+	204	WP_013576617.1	hemolysin expression modulator Hha	Toxin
D3Z09_RS16885	3628302..3629258	-	957	WP_013576618.1	LysR family transcriptional regulator	-
D3Z09_RS16890	3629398..3631185	+	1788	WP_013576619.1	adenine deaminase	-
D3Z09_RS16895	3631262..3632611	+	1350	WP_119261884.1	NCS2 family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8114.45 Da
• Isoelectric Point: 6.9764

>T111608 WP_013576617.1 NZ_CP032296:3628023-3628226 [Rahnella aquatilis]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 204 bp

>T111608 NZ_CP032296:3628023-3628226 [Rahnella aquatilis]
ATGAACAAAATCGACTATTTGATGCGTTTACGAAAATGCACCACTATCGATACACTTGAACGTGTTATTGAAAAAAATAA
GTACGAACTTTCCGACGACGAGCTGGAATTGTTTTACTCGGCAGCTGACCATCGTTTAGCGGAGCTAACGATGAACAAGT
TGTACGATAAAATCCCGGTATCAGTCTGGAAATTTGTACGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14391.15 Da
• Isoelectric Point: 4.7281

>AT111608 WP_013576616.1 NZ_CP032296:3627609-3627977 [Rahnella aquatilis]
MDEYSPKRHDIAQLRYLNEMLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPDDNHLSDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGDYICTLMKT

Nucleotide

• Length: 369 bp

>AT111608 NZ_CP032296:3627609-3627977 [Rahnella aquatilis]
ATGGATGAGTACTCGCCTAAGCGGCACGATATCGCTCAGTTACGCTACCTGAATGAAATGCTGTATGATGAAGGGATTGC
GACACTGGGTGACAGTCATCATGGTTGGGTTAACGATCCGACTTCGGCTGTTAACTTGCAGCTCAATGAACTGATTGAGC
ACATTGCATCCTTTGTGATGAGTTTTAAAATTAAGTATCCAGACGATAACCATTTAAGTGATCTGGTTGAAGAGTATCTG
GATGATACCTACACGCTGTTCAGTAATTATGGAATTAACGATTCTGATTTACGCCAATGGCAAAAGACCAAGAAGCGGTT
ATTCAGAATGTTCTCAGGGGACTACATCTGTACCCTAATGAAGACTTGA

Structures

Toxin

• AlphaFold DB: H8NYI7

Antitoxin

• AlphaFold DB: H8NYI6