Detailed information of TA system TA111596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 814896..815531
• Replicon: chromosome
• Accession: NZ_CP032296
• Organism: Rahnella aquatilis strain ZF7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H8NSH0
• Locus tag: D3Z09_RS03855
• Protein ID: WP_013574084.1
• Coordinates: 815349..815531 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: H8NSG9
• Locus tag: D3Z09_RS03850
• Protein ID: WP_013574083.1
• Coordinates: 814896..815327 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3Z09_RS03825	810105..811559	-	1455	WP_119261264.1	efflux transporter outer membrane subunit	-
D3Z09_RS03830	811583..812104	-	522	WP_013574079.1	transcriptional repressor MprA	-
D3Z09_RS03835	812279..812617	-	339	WP_013574080.1	L-valine transporter subunit YgaH	-
D3Z09_RS03840	812607..813368	-	762	WP_013574081.1	AzlC family ABC transporter permease	-
D3Z09_RS03845	813543..814727	-	1185	WP_119261265.1	MFS transporter	-
D3Z09_RS03850	814896..815327	-	432	WP_013574083.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D3Z09_RS03855	815349..815531	-	183	WP_013574084.1	type II toxin-antitoxin system HicA family toxin	Toxin
D3Z09_RS03860	815648..816643	-	996	WP_013574085.1	glycine betaine/L-proline ABC transporter substrate-binding protein ProX	-
D3Z09_RS03865	816668..817678	-	1011	WP_112152327.1	glycine betaine/L-proline ABC transporter substrate-binding protein ProX	-
D3Z09_RS03870	817784..818908	-	1125	WP_187514584.1	glycine betaine/L-proline ABC transporter permease ProW	-
D3Z09_RS03875	818980..820176	-	1197	WP_013574088.1	glycine betaine/L-proline ABC transporter ATP-binding protein ProV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	741821..846153	104332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-137)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6646.89 Da
• Isoelectric Point: 11.1595

>T111596 WP_013574084.1 NZ_CP032296:c815531-815349 [Rahnella aquatilis]
MKSTELIKALLAAGCELKRHRGGSHQIWWSPVTNKTFPVPHPKNDLPVGTLKSIRKMAGI

Nucleotide

• Length: 183 bp

>T111596 NZ_CP032296:c815531-815349 [Rahnella aquatilis]
ATGAAATCAACGGAATTGATTAAAGCGCTGCTTGCAGCGGGTTGTGAACTCAAGCGTCACCGGGGAGGAAGTCATCAAAT
TTGGTGGTCACCTGTCACCAACAAAACCTTTCCCGTTCCCCACCCCAAAAATGATTTACCTGTGGGAACTCTCAAATCCA
TTAGAAAAATGGCAGGGATATGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 16317.52 Da
• Isoelectric Point: 4.4314

>AT111596 WP_013574083.1 NZ_CP032296:c815327-814896 [Rahnella aquatilis]
MFFSVGLETPKDDKTAYGMVVPAFTAFDYGCFSAADTREEIAPMVREAILMTVEDLIESERFVVDDIFDAGHLVYAANPE
YADFDTWFLIDIDLSEFEGKAHRINISLPDTLIKRIDNKVKNHPSYRDRSHFLAMAARKELLT

Nucleotide

• Length: 432 bp

>AT111596 NZ_CP032296:c815327-814896 [Rahnella aquatilis]
ATGTTCTTTTCAGTCGGTCTTGAAACACCAAAAGATGACAAAACCGCTTACGGTATGGTGGTACCTGCATTTACCGCGTT
TGATTACGGATGCTTCTCTGCAGCGGATACCCGTGAAGAAATCGCCCCTATGGTTCGCGAAGCCATCCTGATGACAGTAG
AAGATTTGATCGAAAGCGAACGCTTTGTTGTTGACGATATTTTTGATGCAGGCCATTTGGTGTATGCCGCTAATCCCGAA
TATGCCGACTTCGATACCTGGTTTTTGATTGATATTGATCTCTCGGAATTCGAAGGGAAAGCGCATCGAATCAATATTTC
GCTGCCTGACACCCTGATCAAACGCATTGATAATAAAGTTAAAAATCATCCGTCATATCGCGACCGCAGCCATTTTCTGG
CGATGGCCGCACGTAAAGAATTGCTGACCTGA

Structures

Toxin

• AlphaFold DB: H8NSH0

Antitoxin

• AlphaFold DB: H8NSG9