Detailed information of TA system TA111589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 352573..353161
• Replicon: plasmid pOXA58_010055
• Accession: NZ_CP032285
• Organism: Acinetobacter sp. WCHA55

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: CDG62_RS02760
• Protein ID: WP_000438825.1
• Coordinates: 352874..353161 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: CDG62_RS02755
• Protein ID: WP_004728120.1
• Coordinates: 352573..352887 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG62_RS02730	348313..349197	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
CDG62_RS02735	349253..350728	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
CDG62_RS02740	351221..351502	-	282	WP_000985609.1	putative addiction module antidote protein	-
CDG62_RS02745	351503..351805	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDG62_RS02750	351954..352484	+	531	WP_000172759.1	hypothetical protein	-
CDG62_RS02755	352573..352887	-	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
CDG62_RS02760	352874..353161	-	288	WP_000438825.1	BrnT family toxin	Toxin
CDG62_RS02765	353391..353996	-	606	WP_000447788.1	LysE family translocator	-
CDG62_RS02770	354069..354682	-	614	Protein_348	IS4 family transposase	-
CDG62_RS02775	354745..355877	-	1133	Protein_349	IS3-like element ISAba14 family transposase	-
CDG62_RS02780	355968..356444	-	477	Protein_350	IS4-like element ISAba1 family transposase	-
CDG62_RS02785	356520..357347	-	828	WP_000073658.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / cmlB1 / mph(E) / msr(E) / blaOXA-58	-	1..372328	372328
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	345092..364862	19770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T111589 WP_000438825.1 NZ_CP032285:c353161-352874 [Acinetobacter sp. WCHA55]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T111589 NZ_CP032285:c353161-352874 [Acinetobacter sp. WCHA55]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT111589 WP_004728120.1 NZ_CP032285:c352887-352573 [Acinetobacter sp. WCHA55]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT111589 NZ_CP032285:c352887-352573 [Acinetobacter sp. WCHA55]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9