Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 7392..7977 Replicon plasmid p5_010034
Accession NZ_CP032273
Organism Acinetobacter sp. WCHAc010034

Toxin (Protein)


Gene name PumA Uniprot ID A0A3B7PW78
Locus tag BEN74_RS00705 Protein ID WP_068912097.1
Coordinates 7675..7977 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A3B7PPK4
Locus tag BEN74_RS00700 Protein ID WP_068912099.1
Coordinates 7392..7673 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN74_RS00665 3455..3772 - 318 WP_068912112.1 hypothetical protein -
BEN74_RS00670 3825..4139 - 315 WP_068912110.1 helix-turn-helix domain-containing protein -
BEN74_RS00675 4120..4443 - 324 WP_000288001.1 hypothetical protein -
BEN74_RS00680 4587..4892 + 306 WP_068912107.1 hypothetical protein -
BEN74_RS00685 4981..5406 + 426 WP_068912106.1 hypothetical protein -
BEN74_RS00690 5534..6127 - 594 WP_068912104.1 hypothetical protein -
BEN74_RS00695 6419..7276 - 858 WP_068912101.1 hypothetical protein -
BEN74_RS00700 7392..7673 - 282 WP_068912099.1 putative addiction module antidote protein Antitoxin
BEN74_RS00705 7675..7977 - 303 WP_068912097.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BEN74_RS00715 8197..8307 - 111 Protein_11 transcriptional regulator -
BEN74_RS00720 8317..8471 + 155 Protein_12 helix-turn-helix domain-containing protein -
BEN74_RS00725 8961..9518 - 558 WP_086374407.1 hypothetical protein -
BEN74_RS00730 9729..10262 - 534 WP_068912119.1 plasmid replication DNA-binding protein -
BEN74_RS00735 10347..11264 - 918 WP_068912117.1 replication initiation protein RepM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11378 11378


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Uncharacterized protein HI1420

(4-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11601.13 Da        Isoelectric Point: 4.9008

>T111583 WP_068912097.1 NZ_CP032273:c7977-7675 [Acinetobacter sp. WCHAc010034]
MYSIYTTEVFDEWFSKLKDQQARRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRVYYCKQGQRIVILLAGGDKS
TQSKDIKLALKLAQDLEEEL

Download         Length: 303 bp

>T111583 NZ_CP032273:c7977-7675 [Acinetobacter sp. WCHAc010034]
ATGTACTCAATATATACCACTGAAGTCTTTGATGAATGGTTTAGCAAGTTAAAAGACCAACAGGCAAGAAGACGCATACA
AGTTCGAATTGATCGGGTAGAAGATGGAAACTTTGGAGATACAGAACCAGTCGGTGAAGGTGTCTCAGAATTACGTTTCT
TCTTTGGACCTGGCTATCGAGTTTATTACTGCAAGCAAGGGCAAAGAATCGTTATTCTCTTAGCTGGTGGAGACAAATCT
ACTCAAAGTAAAGATATAAAACTTGCCCTGAAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10095.68 Da        Isoelectric Point: 8.0002

>AT111583 WP_068912099.1 NZ_CP032273:c7673-7392 [Acinetobacter sp. WCHAc010034]
MAIKLRKWDSAEHLKTEEDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSKDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Download         Length: 282 bp

>AT111583 NZ_CP032273:c7673-7392 [Acinetobacter sp. WCHAc010034]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAACACCTTAAAACAGAAGAAGATATGCAAGCTTATCTACAAGCATG
TATTGAAGAATCTAATGGTGATGCAGCATTCATTGCAAAAGCTTTAGGCAATATCGCTAAAGCTAAAGGAATGGCTCAAT
TATCAAAAGACACAGGTTTAGGCAGAGAAAGTCTTTATAAAGCTCTTTCTGGTGATGTAAACCCAAGCTTTGATACTGTT
ATTAAAGTCGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3B7PW78

Antitoxin

Source ID Structure
AlphaFold DB A0A3B7PPK4

References