Detailed information of TA system TA111582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 262..852
• Replicon: plasmid p4_010034
• Accession: NZ_CP032272
• Organism: Acinetobacter sp. WCHAc010034

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N8YGE2
• Locus tag: BEN74_RS00595
• Protein ID: WP_004787583.1
• Coordinates: 556..852 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BEN74_RS00590
• Protein ID: WP_004787581.1
• Coordinates: 262..552 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN74_RS00590	262..552	-	291	WP_004787581.1	putative addiction module antidote protein	Antitoxin
BEN74_RS00595	556..852	-	297	WP_004787583.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN74_RS00600	1176..1817	-	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
BEN74_RS00605	1883..3070	+	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
BEN74_RS00610	3295..3582	-	288	WP_004728627.1	hypothetical protein	-
BEN74_RS00615	3579..4529	-	951	WP_004728629.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39)	-	1..12457	12457

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Uncharacterized protein HI1420 (9-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11598.27 Da
• Isoelectric Point: 6.4770

>T111582 WP_004787583.1 NZ_CP032272:c852-556 [Acinetobacter sp. WCHAc010034]
MTEIKRLPEFDEWLDSIKDSMTRLKLNRRLDKVQRGNWGDVKHLQEGVWEMREFFGPGWRMYYIKHGDVIIVMLGGGDKS
TQQKDIERAVDLSKSLED

Nucleotide

• Length: 297 bp

>T111582 NZ_CP032272:c852-556 [Acinetobacter sp. WCHAc010034]
ATGACCGAGATTAAACGTCTCCCTGAATTTGATGAATGGCTAGATAGCATTAAAGACAGTATGACCCGTCTTAAACTGAA
TCGAAGATTAGATAAGGTTCAGCGTGGTAATTGGGGCGATGTAAAGCATCTTCAAGAAGGTGTTTGGGAAATGCGAGAGT
TCTTCGGACCAGGTTGGCGCATGTATTACATCAAACATGGTGATGTAATCATTGTGATGCTTGGAGGTGGTGATAAATCT
ACACAGCAAAAGGATATAGAACGAGCTGTTGATTTATCAAAATCTTTAGAGGATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10485.15 Da
• Isoelectric Point: 4.8096

>AT111582 WP_004787581.1 NZ_CP032272:c552-262 [Acinetobacter sp. WCHAc010034]
MVKIADLPSFDMAEALKSEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQNA

Nucleotide

• Length: 291 bp

>AT111582 NZ_CP032272:c552-262 [Acinetobacter sp. WCHAc010034]
ATGGTTAAAATAGCTGATCTACCTAGCTTTGACATGGCAGAAGCACTTAAGAGTGAAGAAGATATTGTAATGTATCTTAA
TATGGTTCTTGAAGAGAATGACCCTGCTGAACTAGCTCATGCACTTGGTGTAATTGCTAAAGCTCGTGGTATGACTCAAA
TCGCTAAAGAAGCAGGAATTGGACGTGAAGCACTTTACAAAGCACTCCGTCAAGATTCAGCACCTCGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGTCTAAAACTAACTGTGCAGAATGCATAA

Structures

Toxin

• AlphaFold DB: A0A6C0XPU5