Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2915017..2915852 Replicon chromosome
Accession NZ_CP032265
Organism Escherichia coli strain AR_0089

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2Y8M938
Locus tag AM468_RS16345 Protein ID WP_047661875.1
Coordinates 2915017..2915394 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2Y0C7M1
Locus tag AM468_RS16350 Protein ID WP_047661876.1
Coordinates 2915484..2915852 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM468_RS16310 2910436..2910678 - 243 Protein_2713 IS110 family transposase -
AM468_RS16315 2911260..2912525 - 1266 WP_032171996.1 integrase arm-type DNA-binding domain-containing protein -
AM468_RS16320 2912989..2913315 - 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
AM468_RS16325 2913312..2913575 - 264 WP_001143297.1 type II toxin-antitoxin system ParD family antitoxin -
AM468_RS16330 2913647..2914513 - 867 WP_047661874.1 DUF4942 domain-containing protein -
AM468_RS16335 2914598..2914795 - 198 WP_087891818.1 DUF957 domain-containing protein -
AM468_RS16340 2914823..2915020 - 198 Protein_2719 hypothetical protein -
AM468_RS16345 2915017..2915394 - 378 WP_047661875.1 TA system toxin CbtA family protein Toxin
AM468_RS16350 2915484..2915852 - 369 WP_047661876.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AM468_RS16355 2915902..2916546 - 645 WP_047661877.1 hypothetical protein -
AM468_RS16360 2916561..2916782 - 222 WP_047661878.1 DUF987 domain-containing protein -
AM468_RS16365 2916851..2917327 - 477 WP_041124171.1 RadC family protein -
AM468_RS16370 2917343..2917828 - 486 WP_073527669.1 antirestriction protein -
AM468_RS16375 2917919..2918737 - 819 WP_094284462.1 DUF945 domain-containing protein -
AM468_RS16380 2918827..2919060 - 234 WP_001278287.1 DUF905 family protein -
AM468_RS16385 2919066..2919743 - 678 WP_021546486.1 hypothetical protein -
AM468_RS16390 2919862..2920746 - 885 WP_032216515.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sul2 / tet(B) / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib rfaE / gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / neuD / neuB / neuA / neuC / neuE / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papC / papD / papI / papB / papA / papH / papC / papD / papJ / papK / papE / papF / papG 2731064..2969209 238145


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14195.18 Da        Isoelectric Point: 8.8867

>T111567 WP_047661875.1 NZ_CP032265:c2915394-2915017 [Escherichia coli]
MKTSPDTHVREASCCPSPVTIWQTLLSRLLDQHYGLTLNNTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQFINSIDILRARRATGLMTRSHYRTVNNITRGKHPEAKR

Download         Length: 378 bp

>T111567 NZ_CP032265:c2915394-2915017 [Escherichia coli]
ATGAAAACATCACCCGATACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATAACACTCCATTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTCTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCCGGGTTC
AGCACTTGTCCCCGCTCTCAGTTCATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGTCTGATGACCCGCAG
CCACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13660.30 Da        Isoelectric Point: 5.4561

>AT111567 WP_047661876.1 NZ_CP032265:c2915852-2915484 [Escherichia coli]
VSDSLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKT

Download         Length: 369 bp

>AT111567 NZ_CP032265:c2915852-2915484 [Escherichia coli]
GTGTCAGACTCGCTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGCAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Y8M938

Antitoxin

Source ID Structure
AlphaFold DB A0A2Y0C7M1

References