Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2340911..2341711 Replicon chromosome
Accession NZ_CP032265
Organism Escherichia coli strain AR_0089

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag AM468_RS13285 Protein ID WP_000342452.1
Coordinates 2341184..2341711 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag AM468_RS13280 Protein ID WP_001277107.1
Coordinates 2340911..2341177 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM468_RS13260 2336570..2337238 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
AM468_RS13265 2337231..2338289 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
AM468_RS13270 2338534..2339388 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
AM468_RS13275 2339659..2340762 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
AM468_RS13280 2340911..2341177 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
AM468_RS13285 2341184..2341711 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
AM468_RS13290 2341708..2342091 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
AM468_RS13295 2342514..2343623 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
AM468_RS13300 2343671..2344597 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
AM468_RS13305 2344594..2345871 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
AM468_RS13310 2345868..2346635 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(88-150)

Antitoxin

(8-86)


Sequences


Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T111563 WP_000342452.1 NZ_CP032265:2341184-2341711 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T111563 NZ_CP032265:2341184-2341711 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT111563 WP_001277107.1 NZ_CP032265:2340911-2341177 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT111563 NZ_CP032265:2340911-2341177 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A061K5K9


Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References