Detailed information of TA system TA111560

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 1311447..1312148
• Replicon: chromosome
• Accession: NZ_CP032265
• Organism: Escherichia coli strain AR_0089

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A515RBV3
• Locus tag: AM468_RS08180
• Protein ID: WP_001531265.1
• Coordinates: 1311447..1311782 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AM468_RS08185
• Protein ID: WP_032143152.1
• Coordinates: 1311813..1312148 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM468_RS08150	1308386..1308557	-	172	Protein_1214	integrase	-
AM468_RS08160	1308968..1309765	-	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
AM468_RS08165	1309879..1310154	-	276	WP_001531268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AM468_RS08170	1310158..1310406	-	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
AM468_RS08175	1310483..1311316	-	834	WP_001531266.1	DUF4942 domain-containing protein	-
AM468_RS08180	1311447..1311782	-	336	WP_001531265.1	TA system toxin CbtA family protein	Toxin
AM468_RS08185	1311813..1312148	-	336	WP_032143152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM468_RS08190	1312148..1312621	-	474	WP_001531261.1	DNA repair protein RadC	-
AM468_RS08195	1312651..1313469	-	819	WP_001531260.1	DUF945 domain-containing protein	-
AM468_RS08200	1313705..1314658	-	954	WP_001531259.1	hypothetical protein	-
AM468_RS08205	1315251..1315865	+	615	WP_001531257.1	inovirus Gp2 family protein	-
AM468_RS08210	1315983..1316201	+	219	WP_001531256.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (11-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12867.89 Da
• Isoelectric Point: 9.8953

>T111560 WP_001531265.1 NZ_CP032265:c1311782-1311447 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRRHNTP

Nucleotide

• Length: 336 bp

>T111560 NZ_CP032265:c1311782-1311447 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.48 Da
• Isoelectric Point: 7.1637

>AT111560 WP_032143152.1 NZ_CP032265:c1312148-1311813 [Escherichia coli]
MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT111560 NZ_CP032265:c1312148-1311813 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A515RBV3