Detailed information of TA system TA111550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 99332..99879
• Replicon: plasmid unnamed1
• Accession: NZ_CP032263
• Organism: Escherichia coli strain AR_0089

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: AM468_RS00610
• Protein ID: WP_000969982.1
• Coordinates: 99598..99879 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: AM468_RS00605
• Protein ID: WP_000079941.1
• Coordinates: 99332..99601 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM468_RS27340	94565..95551	-	987	Protein_105	IS4-like element IS421 family transposase	-
AM468_RS27345	95552..96136	-	585	Protein_106	colicin V synthesis protein	-
AM468_RS00595	96111..97385	-	1275	WP_000489607.1	HlyD family secretion protein	-
AM468_RS00605	99332..99601	+	270	WP_000079941.1	hypothetical protein	Antitoxin
AM468_RS00610	99598..99879	+	282	WP_000969982.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM468_RS00615	99925..100773	+	849	WP_001058005.1	3'-5' exonuclease	-
AM468_RS00620	100890..101372	+	483	WP_001311056.1	hypothetical protein	-
AM468_RS00630	101674..102021	+	348	WP_000142437.1	hypothetical protein	-
AM468_RS00640	102580..103615	+	1036	Protein_114	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / iroN / iroE / iroD / iroC / iroB	1..155784	155784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10877.57 Da
• Isoelectric Point: 7.2749

>T111550 WP_000969982.1 NZ_CP032263:99598-99879 [Escherichia coli]
MMEIFWTMLASQDRKCIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T111550 NZ_CP032263:99598-99879 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGTGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT111550 WP_000079941.1 NZ_CP032263:99332-99601 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT111550 NZ_CP032263:99332-99601 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5