Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 88137..88780 Replicon plasmid unnamed1
Accession NZ_CP032263
Organism Escherichia coli strain AR_0089

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag AM468_RS00530 Protein ID WP_001044768.1
Coordinates 88137..88553 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag AM468_RS00535 Protein ID WP_001261287.1
Coordinates 88550..88780 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM468_RS00505 83241..83525 + 285 WP_012006537.1 ribbon-helix-helix domain-containing protein -
AM468_RS00510 83525..83800 + 276 WP_000421266.1 type II toxin-antitoxin system RelE/ParE family toxin -
AM468_RS00515 83906..84145 + 240 WP_001105068.1 hypothetical protein -
AM468_RS00525 85837..87975 + 2139 WP_000350635.1 AAA family ATPase -
AM468_RS00530 88137..88553 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
AM468_RS00535 88550..88780 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM468_RS00545 89342..89692 + 351 WP_000493379.1 hypothetical protein -
AM468_RS00550 89736..90425 + 690 WP_000796229.1 hypothetical protein -
AM468_RS00555 90422..91213 + 792 WP_000016494.1 site-specific integrase -
AM468_RS00560 91391..91744 + 354 WP_000864812.1 colicin M immunity protein -
AM468_RS00565 91794..92609 - 816 WP_001312845.1 lipid II-degrading bacteriocin colicin M -
AM468_RS00575 92853..93380 + 528 WP_000203272.1 colicin B immunity protein -
AM468_RS00580 93398..93565 - 168 Protein_103 colicin-B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - vat / iroN / iroE / iroD / iroC / iroB 1..155784 155784
- inside IScluster/Tn - - 67664..84883 17219


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T111549 WP_001044768.1 NZ_CP032263:c88553-88137 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T111549 NZ_CP032263:c88553-88137 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT111549 WP_001261287.1 NZ_CP032263:c88780-88550 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT111549 NZ_CP032263:c88780-88550 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References