Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 83241..83800 Replicon plasmid unnamed1
Accession NZ_CP032263
Organism Escherichia coli strain AR_0089

Toxin (Protein)


Gene name relE Uniprot ID A0A0H2XJR9
Locus tag AM468_RS00510 Protein ID WP_000421266.1
Coordinates 83525..83800 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0H2XL20
Locus tag AM468_RS00505 Protein ID WP_012006537.1
Coordinates 83241..83525 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM468_RS00475 78433..79470 - 1038 WP_000110580.1 iron ABC transporter permease -
AM468_RS00480 79470..80468 - 999 WP_000756335.1 ABC transporter substrate-binding protein -
AM468_RS00485 80618..80776 - 159 Protein_87 integrase core domain-containing protein -
AM468_RS00490 81054..82058 - 1005 WP_000427619.1 IS110-like element IS5075 family transposase -
AM468_RS00495 82150..82740 - 591 WP_000086535.1 recombinase family protein -
AM468_RS00505 83241..83525 + 285 WP_012006537.1 ribbon-helix-helix domain-containing protein Antitoxin
AM468_RS00510 83525..83800 + 276 WP_000421266.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM468_RS00515 83906..84145 + 240 WP_001105068.1 hypothetical protein -
AM468_RS00525 85837..87975 + 2139 WP_000350635.1 AAA family ATPase -
AM468_RS00530 88137..88553 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
AM468_RS00535 88550..88780 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - vat / iroN / iroE / iroD / iroC / iroB 1..155784 155784
- inside IScluster/Tn - - 67664..84883 17219


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10779.47 Da        Isoelectric Point: 9.9324

>T111548 WP_000421266.1 NZ_CP032263:83525-83800 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAATRTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T111548 NZ_CP032263:83525-83800 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCACCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10501.96 Da        Isoelectric Point: 6.4632

>AT111548 WP_012006537.1 NZ_CP032263:83241-83525 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT111548 NZ_CP032263:83241-83525 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCAATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGAAATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2XJR9

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2XL20

References