Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 45937..46472 Replicon plasmid unnamed1
Accession NZ_CP032263
Organism Escherichia coli strain AR_0089

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag AM468_RS00310 Protein ID WP_000222766.1
Coordinates 46185..46472 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag AM468_RS00305 Protein ID WP_001132900.1
Coordinates 45937..46188 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM468_RS00255 41064..41624 + 561 WP_000139315.1 fertility inhibition protein FinO -
AM468_RS00260 41760..41972 + 213 WP_001299730.1 ANR family transcriptional regulator -
AM468_RS27335 42218..42292 + 75 Protein_49 endonuclease -
- 42548..42609 - 62 NuclAT_1 - -
- 42548..42609 - 62 NuclAT_1 - -
- 42548..42609 - 62 NuclAT_1 - -
- 42548..42609 - 62 NuclAT_1 - -
AM468_RS00270 42596..42802 + 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
AM468_RS00275 43086..43343 + 258 WP_000083845.1 replication regulatory protein RepA -
AM468_RS00285 43575..43649 + 75 WP_001442103.1 RepA leader peptide Tap -
AM468_RS00290 43642..44124 + 483 WP_001273588.1 hypothetical protein -
AM468_RS00295 44117..44974 + 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
AM468_RS00305 45937..46188 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AM468_RS00310 46185..46472 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - vat / iroN / iroE / iroD / iroC / iroB 1..155784 155784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T111546 WP_000222766.1 NZ_CP032263:46185-46472 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T111546 NZ_CP032263:46185-46472 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT111546 WP_001132900.1 NZ_CP032263:45937-46188 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT111546 NZ_CP032263:45937-46188 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References