Detailed information of TA system TA111528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4323435..4324249
• Replicon: chromosome
• Accession: NZ_CP032261
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: AM446_RS23950
• Protein ID: WP_001054376.1
• Coordinates: 4323435..4323692 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: AM446_RS23955
• Protein ID: WP_001309181.1
• Coordinates: 4323704..4324249 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS23920	4318723..4319829	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
AM446_RS23925	4319894..4320874	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
AM446_RS23930	4321457..4322697	-	1241	Protein_4094	DNA helicase	-
AM446_RS23940	4322813..4323058	+	246	Protein_4095	GNAT family N-acetyltransferase	-
AM446_RS23950	4323435..4323692	+	258	WP_001054376.1	hypothetical protein	Toxin
AM446_RS23955	4323704..4324249	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
AM446_RS23960	4324305..4325051	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
AM446_RS23965	4325220..4325438	+	219	Protein_4099	hypothetical protein	-
AM446_RS28845	4325476..4325592	+	117	Protein_4100	VOC family protein	-
AM446_RS23970	4325837..4326958	+	1122	WP_000010829.1	M42 family metallopeptidase	-
AM446_RS23975	4326955..4327233	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
AM446_RS23980	4327245..4328558	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4315929..4332473	16544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T111528 WP_001054376.1 NZ_CP032261:4323435-4323692 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T111528 NZ_CP032261:4323435-4323692 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT111528 WP_001309181.1 NZ_CP032261:4323704-4324249 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT111528 NZ_CP032261:4323704-4324249 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8