Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3708378..3708996 Replicon chromosome
Accession NZ_CP032261
Organism Escherichia coli strain AR_0067

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM446_RS20890 Protein ID WP_001291435.1
Coordinates 3708778..3708996 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM446_RS20885 Protein ID WP_000344800.1
Coordinates 3708378..3708752 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM446_RS20875 3703467..3704660 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM446_RS20880 3704683..3707832 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
AM446_RS20885 3708378..3708752 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM446_RS20890 3708778..3708996 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM446_RS20895 3709168..3709719 + 552 WP_001522034.1 maltose O-acetyltransferase -
AM446_RS20900 3709835..3710305 + 471 WP_001490888.1 YlaC family protein -
AM446_RS20905 3710469..3712019 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM446_RS20910 3712061..3712414 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AM446_RS20920 3712793..3713104 + 312 WP_000409911.1 MGMT family protein -
AM446_RS20925 3713135..3713707 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111524 WP_001291435.1 NZ_CP032261:3708778-3708996 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111524 NZ_CP032261:3708778-3708996 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT111524 WP_000344800.1 NZ_CP032261:3708378-3708752 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111524 NZ_CP032261:3708378-3708752 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References