Detailed information of TA system TA111513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2534395..2534921
• Replicon: chromosome
• Accession: NZ_CP032261
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AM446_RS14735
• Protein ID: WP_000323025.1
• Coordinates: 2534395..2534682 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AM446_RS14740
• Protein ID: WP_000534858.1
• Coordinates: 2534682..2534921 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS14690	2529479..2529937	-	459	WP_000526135.1	IS200/IS605 family transposase	-
AM446_RS14700	2530388..2530603	-	216	WP_000066484.1	cold shock-like protein CspB	-
AM446_RS14705	2530904..2531116	+	213	WP_000087756.1	cold shock-like protein CspF	-
AM446_RS28635	2531171..2531260	+	90	WP_120795389.1	hypothetical protein	-
AM446_RS14710	2531538..2532290	-	753	WP_001047135.1	antitermination protein	-
AM446_RS14715	2532304..2533353	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
AM446_RS14720	2533355..2533633	-	279	WP_012304870.1	hypothetical protein	-
AM446_RS14725	2533700..2533951	-	252	WP_000980994.1	hypothetical protein	-
AM446_RS14730	2534168..2534323	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
AM446_RS14735	2534395..2534682	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AM446_RS14740	2534682..2534921	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AM446_RS14745	2534946..2535251	+	306	WP_001326990.1	hypothetical protein	-
AM446_RS14750	2535454..2535786	+	333	WP_001301033.1	protein FlxA	-
AM446_RS14755	2536223..2537536	-	1314	WP_119163948.1	ISNCY family transposase	-
AM446_RS14760	2538020..2539048	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
AM446_RS14765	2539045..2539659	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2501502..2549439	47937
	-	flank	IS/Tn	-	-	2529479..2529937	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T111513 WP_000323025.1 NZ_CP032261:c2534682-2534395 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T111513 NZ_CP032261:c2534682-2534395 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT111513 WP_000534858.1 NZ_CP032261:c2534921-2534682 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT111513 NZ_CP032261:c2534921-2534682 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8