Detailed information of TA system TA111512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2441544..2442182
• Replicon: chromosome
• Accession: NZ_CP032261
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: AM446_RS14240
• Protein ID: WP_001306887.1
• Coordinates: 2442006..2442182 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM446_RS14235
• Protein ID: WP_001270286.1
• Coordinates: 2441544..2441960 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS14215	2436696..2437637	-	942	WP_119163947.1	ABC transporter permease	-
AM446_RS14220	2437638..2438651	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
AM446_RS14225	2438669..2439814	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
AM446_RS14230	2440059..2441465	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
AM446_RS14235	2441544..2441960	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AM446_RS14240	2442006..2442182	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AM446_RS14245	2442404..2442634	+	231	WP_000494241.1	YncJ family protein	-
AM446_RS14250	2442726..2444687	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AM446_RS14255	2444760..2445296	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
AM446_RS14260	2445349..2446560	+	1212	WP_023143671.1	BenE family transporter YdcO	-
AM446_RS14265	2446668..2447126	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2446668..2447126	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T111512 WP_001306887.1 NZ_CP032261:c2442182-2442006 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T111512 NZ_CP032261:c2442182-2442006 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT111512 WP_001270286.1 NZ_CP032261:c2441960-2441544 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT111512 NZ_CP032261:c2441960-2441544 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76