Detailed information of TA system TA111498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 58096..58655
• Replicon: plasmid unnamed3
• Accession: NZ_CP032260
• Organism: Escherichia coli strain AR_0067

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AM446_RS01545
• Protein ID: WP_049143443.1
• Coordinates: 58380..58655 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AM446_RS01540
• Protein ID: WP_049143442.1
• Coordinates: 58096..58380 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM446_RS01495	53125..53745	-	621	WP_000176497.1	DUF1449 family protein	-
AM446_RS01505	54012..54674	-	663	WP_000154142.1	hypothetical protein	-
AM446_RS01510	54815..55456	-	642	WP_000335849.1	transcription termination factor NusG	-
AM446_RS01525	56157..57188	+	1032	WP_000907880.1	replication initiation protein	-
AM446_RS01540	58096..58380	+	285	WP_049143442.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM446_RS01545	58380..58655	+	276	WP_049143443.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM446_RS01550	58761..59042	+	282	WP_001105066.1	hypothetical protein	-
AM446_RS28785	59233..59499	-	267	WP_000627831.1	hypothetical protein	-
AM446_RS28790	59499..59681	-	183	Protein_60	hypothetical protein	-
AM446_RS01570	59737..60339	-	603	WP_000517694.1	hypothetical protein	-
AM446_RS01580	60693..62039	+	1347	WP_001132022.1	hypothetical protein	-
AM446_RS28465	62550..62852	-	303	WP_001245884.1	hypothetical protein	-
AM446_RS01595	62849..63475	-	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..87423	87423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T111498 WP_049143443.1 NZ_CP032260:58380-58655 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T111498 NZ_CP032260:58380-58655 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10504.92 Da
• Isoelectric Point: 5.6558

>AT111498 WP_049143442.1 NZ_CP032260:58096-58380 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT111498 NZ_CP032260:58096-58380 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA