Detailed information of TA system TA111478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpB-mazE/PRK09812-ChpS
• Location: 25437..26032
• Replicon: plasmid unnamed
• Accession: NZ_CP032256
• Organism: Pseudomonas aeruginosa strain AR_0111

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A2K4Y2Z0
• Locus tag: AM490_RS00195
• Protein ID: WP_023100676.1
• Coordinates: 25437..25787 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2K4Y2Y6
• Locus tag: AM490_RS00200
• Protein ID: WP_023100677.1
• Coordinates: 25784..26032 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM490_RS00155	21139..21630	+	492	WP_023100670.1	hypothetical protein	-
AM490_RS00160	21862..22605	+	744	WP_023100671.1	hypothetical protein	-
AM490_RS00165	22929..23192	+	264	WP_020303033.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
AM490_RS00170	23179..23460	+	282	WP_023100672.1	type II toxin-antitoxin system YafQ family toxin	-
AM490_RS00175	23497..23832	+	336	WP_023100673.1	hypothetical protein	-
AM490_RS00185	24214..24666	+	453	WP_023100674.1	hypothetical protein	-
AM490_RS00190	24715..25437	-	723	WP_031634065.1	hypothetical protein	-
AM490_RS00195	25437..25787	-	351	WP_023100676.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AM490_RS00200	25784..26032	-	249	WP_023100677.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM490_RS34080	26029..26448	-	420	WP_041167413.1	hypothetical protein	-
AM490_RS00205	26568..27044	-	477	WP_023100690.1	single-stranded DNA-binding protein	-
AM490_RS00210	27055..27492	-	438	WP_023100689.1	hypothetical protein	-
AM490_RS00215	27501..28184	-	684	WP_023100688.1	hypothetical protein	-
AM490_RS00220	28181..28717	-	537	WP_023100687.1	hypothetical protein	-
AM490_RS00225	28714..28944	-	231	WP_023100686.1	hypothetical protein	-
AM490_RS00230	28934..31003	-	2070	WP_023100685.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..129422	129422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-112)

Antitoxin

• Antitoxin PemI-like (1-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12073.06 Da
• Isoelectric Point: 10.1005

>T111478 WP_023100676.1 NZ_CP032256:c25787-25437 [Pseudomonas aeruginosa]
MSKRLQHQFDRGDIVSLDMGSASAQAACKALVLSPAAYNVLGLALAIPITEHDSSRYAGFAVAILLPGRTPAKGAALVNL
VRPVDLAARGAKLLGKAPQTTIDEALQRLQAVVGKD

Nucleotide

• Length: 351 bp

>T111478 NZ_CP032256:c25787-25437 [Pseudomonas aeruginosa]
ATGAGCAAACGCCTGCAGCATCAGTTCGACCGCGGCGATATCGTCTCCTTGGACATGGGTAGCGCTTCAGCACAGGCGGC
CTGCAAAGCGCTCGTGCTTTCCCCTGCAGCGTACAACGTCCTGGGGCTCGCCCTAGCCATTCCTATCACCGAGCACGACA
GCAGCCGCTACGCCGGCTTTGCCGTCGCGATCCTCCTTCCAGGGCGCACGCCGGCGAAGGGGGCAGCCTTGGTCAACCTG
GTGCGGCCGGTGGATCTTGCTGCAAGAGGTGCAAAGCTGCTCGGGAAGGCGCCCCAGACGACCATAGATGAAGCCCTGCA
GCGCCTGCAGGCGGTTGTTGGGAAAGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8738.98 Da
• Isoelectric Point: 4.1093

>AT111478 WP_023100677.1 NZ_CP032256:c26032-25784 [Pseudomonas aeruginosa]
MKTTIRAIGNSQGITIPKPLLAELGVSLGDPVDVSFKDQTLVVTKVATEYDLSTLVAQCDLEAPEPPDIQCWRTATPVGN
EA

Nucleotide

• Length: 249 bp

>AT111478 NZ_CP032256:c26032-25784 [Pseudomonas aeruginosa]
TTGAAAACGACTATCCGTGCGATTGGGAACTCTCAGGGCATCACAATACCCAAGCCGTTGCTGGCCGAACTCGGCGTAAG
CCTTGGCGACCCTGTAGACGTCTCCTTCAAAGACCAGACCTTGGTGGTGACCAAGGTCGCCACCGAATATGACCTCAGCA
CGCTAGTCGCCCAGTGTGACCTGGAGGCCCCGGAGCCGCCGGACATCCAATGCTGGAGAACAGCTACGCCGGTAGGAAAC
GAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A2K4Y2Z0

Antitoxin

• AlphaFold DB: A0A2K4Y2Y6