Detailed information of TA system TA111477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 22929..23460
• Replicon: plasmid unnamed
• Accession: NZ_CP032256
• Organism: Pseudomonas aeruginosa strain AR_0111

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2K4Y311
• Locus tag: AM490_RS00170
• Protein ID: WP_023100672.1
• Coordinates: 23179..23460 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: W2D9V6
• Locus tag: AM490_RS00165
• Protein ID: WP_020303033.1
• Coordinates: 22929..23192 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM490_RS00140	18623..18940	+	318	WP_171947059.1	hypothetical protein	-
AM490_RS00145	19133..19594	+	462	WP_031634057.1	hypothetical protein	-
AM490_RS34075	19587..19763	+	177	WP_153549643.1	hypothetical protein	-
AM490_RS00150	19781..21139	+	1359	WP_023100669.1	replicative DNA helicase	-
AM490_RS00155	21139..21630	+	492	WP_023100670.1	hypothetical protein	-
AM490_RS00160	21862..22605	+	744	WP_023100671.1	hypothetical protein	-
AM490_RS00165	22929..23192	+	264	WP_020303033.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AM490_RS00170	23179..23460	+	282	WP_023100672.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AM490_RS00175	23497..23832	+	336	WP_023100673.1	hypothetical protein	-
AM490_RS00185	24214..24666	+	453	WP_023100674.1	hypothetical protein	-
AM490_RS00190	24715..25437	-	723	WP_031634065.1	hypothetical protein	-
AM490_RS00195	25437..25787	-	351	WP_023100676.1	type II toxin-antitoxin system PemK/MazF family toxin	-
AM490_RS00200	25784..26032	-	249	WP_023100677.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AM490_RS34080	26029..26448	-	420	WP_041167413.1	hypothetical protein	-
AM490_RS00205	26568..27044	-	477	WP_023100690.1	single-stranded DNA-binding protein	-
AM490_RS00210	27055..27492	-	438	WP_023100689.1	hypothetical protein	-
AM490_RS00215	27501..28184	-	684	WP_023100688.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..129422	129422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10724.19 Da
• Isoelectric Point: 9.0277

>T111477 WP_023100672.1 NZ_CP032256:23179-23460 [Pseudomonas aeruginosa]
MRTIEQTSRFKRDYKREAKGPHRQTLASDFVAIVTALANDQPLAEKHRDHALNGDWKDHRDCHIKPDLVLIYRKPDDAVL
QLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T111477 NZ_CP032256:23179-23460 [Pseudomonas aeruginosa]
ATGCGGACGATTGAACAGACCAGCCGGTTCAAGCGCGATTACAAACGCGAAGCCAAAGGCCCGCATCGTCAGACGCTGGC
GAGCGATTTCGTCGCCATTGTCACAGCCCTTGCGAATGACCAGCCGCTGGCCGAGAAGCACCGCGACCACGCGCTCAATG
GCGACTGGAAAGATCACCGGGACTGCCACATCAAGCCTGACCTGGTGCTGATTTACCGCAAGCCGGATGACGCCGTGTTG
CAACTCGTGCGTCTCGGCTCGCATAGCGAGCTTGGCCTGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9471.95 Da
• Isoelectric Point: 4.8096

>AT111477 WP_020303033.1 NZ_CP032256:22929-23192 [Pseudomonas aeruginosa]
MSANAVVRARIDEHIKEEATVVLAAMGLTVSDAFRIMLTRVAREKALPFEPLIPNATTIEAMKEARRGGLKSFASVEDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT111477 NZ_CP032256:22929-23192 [Pseudomonas aeruginosa]
ATGTCTGCCAATGCTGTAGTCCGTGCCCGTATCGACGAGCACATCAAGGAAGAGGCGACCGTCGTGCTGGCGGCGATGGG
GCTCACCGTGTCCGACGCCTTCCGCATCATGTTGACCCGCGTCGCCCGCGAGAAAGCCTTGCCGTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCGCGCCGTGGTGGCCTGAAGTCGTTTGCTTCCGTCGAGGACCTGATG
GCGGACCTGAATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A2K4Y311

Antitoxin

• AlphaFold DB: A0A0W0N2R7