Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 44083..44726 Replicon plasmid unnamed2
Accession NZ_CP032242
Organism Klebsiella pneumoniae strain XJ-K2

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag D3N36_RS28160 Protein ID WP_001044770.1
Coordinates 44083..44499 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag D3N36_RS28165 Protein ID WP_001261282.1
Coordinates 44496..44726 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3N36_RS28140 40186..40458 - 273 Protein_44 transposase -
D3N36_RS28145 40487..41184 - 698 Protein_45 IS1 family transposase -
D3N36_RS28150 41440..42462 - 1023 WP_000361404.1 helicase UvrD -
D3N36_RS28155 42447..44009 - 1563 WP_004206609.1 AAA family ATPase -
D3N36_RS28160 44083..44499 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
D3N36_RS28165 44496..44726 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D3N36_RS29665 44683..45144 + 462 WP_014343465.1 hypothetical protein -
D3N36_RS28170 45305..46249 + 945 WP_011977810.1 hypothetical protein -
D3N36_RS28175 46286..46678 + 393 WP_011977811.1 hypothetical protein -
D3N36_RS28180 46736..47257 + 522 WP_013214008.1 hypothetical protein -
D3N36_RS28185 47303..47506 + 204 WP_011977813.1 hypothetical protein -
D3N36_RS28190 47536..48538 + 1003 Protein_55 hypothetical protein -
D3N36_RS28195 48722..49501 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 - 1..96030 96030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T111472 WP_001044770.1 NZ_CP032242:c44499-44083 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T111472 NZ_CP032242:c44499-44083 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT111472 WP_001261282.1 NZ_CP032242:c44726-44496 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT111472 NZ_CP032242:c44726-44496 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References