Detailed information of TA system TA111470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 78716..79626
• Replicon: plasmid unnamed1
• Accession: NZ_CP032241
• Organism: Klebsiella pneumoniae strain XJ-K2

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: D3N36_RS27235
• Protein ID: WP_004026354.1
• Coordinates: 79156..79626 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: D3N36_RS27230
• Protein ID: WP_004026357.1
• Coordinates: 78716..79159 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3N36_RS27205	74225..75127	+	903	WP_004213613.1	DMT family transporter	-
D3N36_RS27210	75270..75491	-	222	WP_004213615.1	hypothetical protein	-
D3N36_RS27215	75553..76860	-	1308	Protein_89	FepA family TonB-dependent siderophore receptor	-
D3N36_RS27220	76926..77906	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
D3N36_RS27225	78112..78615	+	504	Protein_91	DUF4113 domain-containing protein	-
D3N36_RS27230	78716..79159	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
D3N36_RS27235	79156..79626	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
D3N36_RS27240	79775..80472	+	698	Protein_94	IS1 family transposase	-
D3N36_RS27245	81858..82807	-	950	Protein_95	DUF1173 family protein	-
D3N36_RS27250	82985..83479	+	495	WP_004212794.1	thermonuclease family protein	-
D3N36_RS27255	83816..84214	+	399	WP_004212796.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN	1..219775	219775
	-	inside	IScluster/Tn	-	rmpA / rmpA / iroN	65932..85665	19733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T111470 WP_004026354.1 NZ_CP032241:79156-79626 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T111470 NZ_CP032241:79156-79626 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT111470 WP_004026357.1 NZ_CP032241:78716-79159 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT111470 NZ_CP032241:78716-79159 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9