Detailed information of TA system TA111469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 38897..39567
• Replicon: plasmid unnamed1
• Accession: NZ_CP032241
• Organism: Klebsiella pneumoniae strain XJ-K2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: D3N36_RS26995
• Protein ID: WP_004213072.1
• Coordinates: 39124..39567 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: D3N36_RS26990
• Protein ID: WP_004213073.1
• Coordinates: 38897..39127 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3N36_RS26965	34082..34861	-	780	WP_004213560.1	site-specific integrase	-
D3N36_RS26970	34858..35679	-	822	WP_004213562.1	hypothetical protein	-
D3N36_RS26975	36651..36857	+	207	WP_004213077.1	hypothetical protein	-
D3N36_RS26980	36847..37140	-	294	WP_004213076.1	hypothetical protein	-
D3N36_RS26985	37156..38289	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
D3N36_RS26990	38897..39127	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D3N36_RS26995	39124..39567	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
D3N36_RS27000	39581..39997	-	417	Protein_46	restriction endonuclease	-
D3N36_RS27005	40001..40294	-	294	Protein_47	transposase	-
D3N36_RS29630	40711..41346	+	636	Protein_48	response regulator	-
D3N36_RS27015	41861..42267	-	407	Protein_49	GAF domain-containing protein	-
D3N36_RS27020	42358..43278	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
D3N36_RS27025	43327..43818	-	492	WP_011251327.1	DM13 domain-containing protein	-
D3N36_RS27030	43881..44156	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN	1..219775	219775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T111469 WP_004213072.1 NZ_CP032241:39124-39567 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T111469 NZ_CP032241:39124-39567 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT111469 WP_004213073.1 NZ_CP032241:38897-39127 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT111469 NZ_CP032241:38897-39127 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620