Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 51215..51816 Replicon plasmid pTB222
Accession NZ_CP032239
Organism Escherichia coli strain ECCWS199

Toxin (Protein)


Gene name doc Uniprot ID A0A649Z4R3
Locus tag D3W53_RS25435 Protein ID WP_072647225.1
Coordinates 51436..51816 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag D3W53_RS25430 Protein ID WP_001190712.1
Coordinates 51215..51436 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W53_RS25420 48211..49479 - 1269 WP_072647224.1 restriction endonuclease subunit S -
D3W53_RS25425 49476..51032 - 1557 WP_072647227.1 type I restriction-modification system subunit M -
D3W53_RS25430 51215..51436 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D3W53_RS25435 51436..51816 + 381 WP_072647225.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
D3W53_RS25440 51821..52000 + 180 WP_000113018.1 hypothetical protein -
D3W53_RS25445 52028..53071 + 1044 WP_072647226.1 DUF968 domain-containing protein -
D3W53_RS25450 53195..54163 - 969 WP_000654811.1 IS5 family transposase -
D3W53_RS25455 54332..54451 + 120 WP_071527722.1 ash family protein -
D3W53_RS25460 54470..54691 + 222 WP_001361625.1 host cell division inhibitor Icd-like protein -
D3W53_RS25465 54688..55803 + 1116 WP_032203728.1 phage antirepressor KilAC domain-containing protein -
D3W53_RS25470 55836..56687 - 852 WP_000611664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA2 / dfrA12 / tet(A) / floR / sul2 / blaTEM-1B / sul3 / aph(3')-Ia / mph(A) - 1..159756 159756


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13572.34 Da        Isoelectric Point: 5.6343

>T111449 WP_072647225.1 NZ_CP032239:51436-51816 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELAVLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T111449 NZ_CP032239:51436-51816 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGTCCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT111449 WP_001190712.1 NZ_CP032239:51215-51436 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT111449 NZ_CP032239:51215-51436 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A649Z4R3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References