Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17377..18020 Replicon plasmid pTB222
Accession NZ_CP032239
Organism Escherichia coli strain ECCWS199

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag D3W53_RS25230 Protein ID WP_000754566.1
Coordinates 17377..17793 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag D3W53_RS25235 Protein ID WP_001261276.1
Coordinates 17790..18020 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W53_RS25215 14958..16034 - 1077 WP_000227969.1 IS110 family transposase -
D3W53_RS25225 16316..17172 - 857 Protein_15 IS3 family transposase -
D3W53_RS25230 17377..17793 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
D3W53_RS25235 17790..18020 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D3W53_RS25245 18219..18710 - 492 Protein_18 IS5-like element ISKpn26 family transposase -
D3W53_RS25250 18816..19520 - 705 Protein_19 IS6-like element IS26 family transposase -
D3W53_RS26630 19584..19706 + 123 Protein_20 IS66 family insertion sequence element accessory protein TnpB -
D3W53_RS25260 19798..20579 - 782 Protein_21 IS21-like element helper ATPase IstB -
D3W53_RS25265 20576..21184 - 609 Protein_22 transposase -
D3W53_RS25270 21198..21570 - 373 Protein_23 IS1 family transposase -
D3W53_RS25275 22080..22790 + 711 WP_119173451.1 cache domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA2 / dfrA12 / tet(A) / floR / sul2 / blaTEM-1B / sul3 / aph(3')-Ia / mph(A) - 1..159756 159756
- inside IScluster/Tn aadA2 / dfrA12 - 3455..21280 17825


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T111448 WP_000754566.1 NZ_CP032239:c17793-17377 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T111448 NZ_CP032239:c17793-17377 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT111448 WP_001261276.1 NZ_CP032239:c18020-17790 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT111448 NZ_CP032239:c18020-17790 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References