Detailed information of TA system TA111442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2486196..2486834
• Replicon: chromosome
• Accession: NZ_CP032237
• Organism: Escherichia coli strain ECCWS199

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: D3W53_RS12665
• Protein ID: WP_000813794.1
• Coordinates: 2486658..2486834 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D3W53_RS12660
• Protein ID: WP_001270286.1
• Coordinates: 2486196..2486612 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3W53_RS12640	2481348..2482289	-	942	WP_001251313.1	ABC transporter permease	-
D3W53_RS12645	2482290..2483303	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
D3W53_RS12650	2483321..2484466	-	1146	WP_072778914.1	ABC transporter substrate-binding protein	-
D3W53_RS12655	2484711..2486117	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
D3W53_RS12660	2486196..2486612	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D3W53_RS12665	2486658..2486834	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D3W53_RS12670	2487056..2487286	+	231	WP_000494244.1	YncJ family protein	-
D3W53_RS12675	2487378..2489339	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D3W53_RS12680	2489412..2489948	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
D3W53_RS12685	2490001..2491215	+	1215	WP_071597387.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2491513..2492520	1007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T111442 WP_000813794.1 NZ_CP032237:c2486834-2486658 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T111442 NZ_CP032237:c2486834-2486658 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT111442 WP_001270286.1 NZ_CP032237:c2486612-2486196 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT111442 NZ_CP032237:c2486612-2486196 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB