Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 783102..783900 Replicon chromosome
Accession NZ_CP032237
Organism Escherichia coli strain ECCWS199

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0HL60
Locus tag D3W53_RS03985 Protein ID WP_000854692.1
Coordinates 783102..783479 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1L4NTN5
Locus tag D3W53_RS03990 Protein ID WP_001605875.1
Coordinates 783526..783900 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3W53_RS03955 778229..779377 - 1149 WP_000905931.1 capsule polysaccharide export inner-membrane protein KpsE -
D3W53_RS03960 779449..780432 - 984 WP_001298261.1 KpsF/GutQ family sugar-phosphate isomerase -
D3W53_RS03965 781243..781413 - 171 Protein_734 IS110 family transposase -
D3W53_RS03970 781756..782598 - 843 WP_001529559.1 DUF4942 domain-containing protein -
D3W53_RS03975 782683..782880 - 198 WP_023563519.1 DUF957 domain-containing protein -
D3W53_RS03980 782956..783105 - 150 Protein_737 hypothetical protein -
D3W53_RS03985 783102..783479 - 378 WP_000854692.1 TA system toxin CbtA family protein Toxin
D3W53_RS03990 783526..783900 - 375 WP_001605875.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
D3W53_RS03995 783980..784201 - 222 WP_000692303.1 DUF987 domain-containing protein -
D3W53_RS04000 784270..784746 - 477 WP_001605874.1 RadC family protein -
D3W53_RS04005 784762..785241 - 480 WP_001605873.1 antirestriction protein -
D3W53_RS04010 785323..785658 - 336 Protein_743 DUF932 domain-containing protein -
D3W53_RS04015 785695..785844 + 150 WP_072146520.1 hemolysin activation protein -
D3W53_RS04020 786076..786312 + 237 Protein_745 hypothetical protein -
D3W53_RS04025 786317..787183 - 867 Protein_746 integrase arm-type DNA-binding domain-containing protein -
D3W53_RS04035 787562..788269 - 708 WP_000234526.1 DUF554 domain-containing protein -
D3W53_RS26680 788422..788505 + 84 WP_211180506.1 protein YqgH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14119.12 Da        Isoelectric Point: 7.3249

>T111430 WP_000854692.1 NZ_CP032237:c783479-783102 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWHTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Download         Length: 378 bp

>T111430 NZ_CP032237:c783479-783102 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13594.42 Da        Isoelectric Point: 5.4554

>AT111430 WP_001605875.1 NZ_CP032237:c783900-783526 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT111430 NZ_CP032237:c783900-783526 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0HL60

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4NTN5

References