Detailed information of TA system TA111421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 23466..24376
• Replicon: plasmid unnamed1
• Accession: NZ_CP032223
• Organism: Klebsiella pneumoniae strain AR_0046

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: AM425_RS26270
• Protein ID: WP_060577855.1
• Coordinates: 23466..23936 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: AM425_RS26275
• Protein ID: WP_004026357.1
• Coordinates: 23933..24376 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM425_RS26235	19533..19829	-	297	WP_094284716.1	hydrogenase expression/formation protein HypD	-
AM425_RS26245	20061..20438	+	378	WP_004883386.1	hypothetical protein	-
AM425_RS30490	20636..21610	-	975	WP_004883382.1	hypothetical protein	-
AM425_RS26255	22216..22374	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
AM425_RS26265	23097..23357	+	261	WP_004026352.1	hypothetical protein	-
AM425_RS26270	23466..23936	-	471	WP_060577855.1	RES family NAD+ phosphorylase	Toxin
AM425_RS26275	23933..24376	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
AM425_RS26280	24476..25747	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
AM425_RS26285	25747..26169	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AM425_RS26295	26579..28066	+	1488	WP_162897207.1	group II intron reverse transcriptase/maturase	-
AM425_RS26300	28434..29003	-	570	Protein_28	transposase	-
AM425_RS26305	29005..29220	+	216	Protein_29	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..201149	201149
	-	flank	IS/Tn	-	-	28434..28787	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17442.78 Da
• Isoelectric Point: 4.7424

>T111421 WP_060577855.1 NZ_CP032223:c23936-23466 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMGSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVLKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T111421 NZ_CP032223:c23936-23466 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGGCTCTTTCACTCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCTGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT111421 WP_004026357.1 NZ_CP032223:c24376-23933 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT111421 NZ_CP032223:c24376-23933 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCCAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACTTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V3Q9