Detailed information of TA system TA111401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 21333..21895
• Replicon: plasmid unnamed4
• Accession: NZ_CP032210
• Organism: Klebsiella pneumoniae strain AR_0109

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: AM488_RS29795
• Protein ID: WP_001623607.1
• Coordinates: 21578..21895 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: AM488_RS29790
• Protein ID: WP_001623606.1
• Coordinates: 21333..21578 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM488_RS29760	17857..18381	+	525	WP_001610342.1	DnaJ domain-containing protein	-
AM488_RS29765	18432..18995	+	564	WP_001610343.1	hypothetical protein	-
AM488_RS29770	19063..19359	+	297	WP_001610345.1	hypothetical protein	-
AM488_RS29775	19343..19846	+	504	WP_001610346.1	hypothetical protein	-
AM488_RS29780	20623..20811	+	189	WP_001610336.1	hypothetical protein	-
AM488_RS31800	20859..21032	+	174	WP_001610337.1	hypothetical protein	-
AM488_RS29790	21333..21578	+	246	WP_001623606.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AM488_RS29795	21578..21895	+	318	WP_001623607.1	CcdB family protein	Toxin
AM488_RS29800	22176..22430	+	255	WP_072010979.1	hypothetical protein	-
AM488_RS29805	22441..22671	+	231	Protein_29	hypothetical protein	-
AM488_RS29810	22689..23084	+	396	WP_097337520.1	hypothetical protein	-
AM488_RS29815	23102..23407	+	306	WP_097337519.1	hypothetical protein	-
AM488_RS29820	23599..24057	-	459	WP_037404219.1	hypothetical protein	-
AM488_RS29825	24622..25386	+	765	WP_037404221.1	tyrosine-type recombinase/integrase	-
AM488_RS29830	25500..26165	+	666	WP_001623616.1	hypothetical protein	-
AM488_RS29835	26211..26531	+	321	WP_037404223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40448	40448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-104)

Antitoxin

• Post-segregation antitoxin CcdA (9-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11782.77 Da
• Isoelectric Point: 8.4499

>T111401 WP_001623607.1 NZ_CP032210:21578-21895 [Klebsiella pneumoniae]
VKQFAVYKNKSRNKQAYPYFIDVQADMLAHLNTRLVMPLTQKANSNSQVKALTPIITIDQIDYVVLATMITTTDVKNLKA
EDAVMDASHLRDQLVSAIDMMILGI

Nucleotide

• Length: 318 bp

>T111401 NZ_CP032210:21578-21895 [Klebsiella pneumoniae]
GTGAAACAGTTTGCAGTTTACAAAAATAAATCCCGGAACAAACAGGCATACCCGTATTTTATTGATGTGCAGGCAGATAT
GCTTGCACACCTTAATACTCGCCTAGTTATGCCTTTAACGCAGAAAGCTAATTCTAATTCGCAGGTAAAAGCGTTAACGC
CAATTATTACAATCGATCAGATCGATTATGTAGTGCTGGCAACCATGATAACGACAACGGATGTAAAAAATCTGAAAGCT
GAAGATGCAGTTATGGATGCTTCACATTTACGCGATCAATTGGTTTCTGCGATTGATATGATGATTTTAGGTATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9198.25 Da
• Isoelectric Point: 4.4243

>AT111401 WP_001623606.1 NZ_CP032210:21333-21578 [Klebsiella pneumoniae]
MRTNVAEKKRSTNVYLNAGLLEEAKELDLNVSAISNQALETAVRERKRERWIEENRAGIEALNSFVEEVGIFSDDENFGV
I

Nucleotide

• Length: 246 bp

>AT111401 NZ_CP032210:21333-21578 [Klebsiella pneumoniae]
ATGAGAACGAATGTAGCCGAGAAAAAACGTAGTACAAACGTGTACTTAAATGCTGGCCTACTTGAAGAGGCGAAAGAGCT
GGACTTGAATGTTTCCGCGATATCTAACCAGGCGCTGGAAACGGCTGTCAGAGAACGCAAGCGTGAACGTTGGATCGAGG
AAAACCGCGCGGGCATCGAGGCGTTAAACAGCTTCGTGGAAGAGGTGGGTATTTTTTCAGATGATGAAAATTTCGGGGTG
ATTTAG