Detailed information of TA system TA111376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 5398228..5399065
• Replicon: chromosome
• Accession: NZ_CP032204
• Organism: Escherichia coli strain AR_0013

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: AM340_RS27850
• Protein ID: WP_000227787.1
• Coordinates: 5398523..5399065 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: AM340_RS27845
• Protein ID: WP_001353405.1
• Coordinates: 5398228..5398539 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM340_RS27820	5393248..5394195	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
AM340_RS27825	5394217..5396208	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AM340_RS27830	5396198..5396812	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AM340_RS27835	5396812..5397141	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AM340_RS27840	5397153..5398043	+	891	WP_000971336.1	heme o synthase	-
AM340_RS27845	5398228..5398539	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
AM340_RS27850	5398523..5399065	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
AM340_RS27855	5399121..5400056	-	936	WP_012311701.1	sel1 repeat family protein	-
AM340_RS27860	5400463..5401827	+	1365	WP_119164947.1	MFS transporter	-
AM340_RS27865	5401955..5402446	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AM340_RS27870	5402614..5403525	+	912	WP_000705836.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T111376 WP_000227787.1 NZ_CP032204:5398523-5399065 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T111376 NZ_CP032204:5398523-5399065 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGTGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT111376 WP_001353405.1 NZ_CP032204:5398228-5398539 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT111376 NZ_CP032204:5398228-5398539 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3